Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-06-05T02:16:29.870Z Has data issue: false hasContentIssue false

Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)

Published online by Cambridge University Press:  20 November 2017

D. Colombatto
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, RG6 6AR, UK FMS Division, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
F. L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, RG6 6AR, UK
M. K. Bhat
Affiliation:
FMS Division, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 237, RG6 6AR, UK
Get access

Extract

Modern feeding practices often lead to ruminal conditions being sub-optimal for fibre digestion. It has been speculated that fibrolytic enzymes, which usually show optimum activity at pH values below 6.0, may be of benefit when applied to diets of high producing animals. This study used a commercial enzyme mixture (EM), already identified as effective; to investigate its optimum pH range with respect to activity and its impact on the fermentation profiles of pure substrates, under differing pH conditions.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Colombatto, D, Mould, F L, Bhat, M K & Owen, E. 2000. Biochemical and in vitro assessment of six enzyme preparations as potential feed additives. Proceedings of the British Society of Animal Science, Page 54.CrossRefGoogle Scholar
Mauricio, R M, Mould, F L, Dhanoa, M S, Owen, E, Channa, K S & Theodorou, M K. 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science And Technology 79: 321330.CrossRefGoogle Scholar
Wood, T M & Bhat, M K. 1988. Methods for measuring cellulase activities. Methods in Enzymology. Wood, W.A. and Kellogg, S.T. (Eds.), Vol. 160: 87112. Academic Press. London, UK.Google Scholar