There is an increased demand for raw materials that can be used as substrate for bio-ethanol production. The resultant by-products will have an important role in animal nutrition as possible feeds. Most of these potential feeds are high in cell walls with high lignin contents, limiting its nutritive value. The development and use of alternative enzyme methodologies can increase the availability of structural carbohydrates. Enzymes with the potential to break down cell walls, including lignin, are available in aerobic white-rot fungi. The aim of this study was to evaluate two white-rot cultivation procedures in wheat straw in order to determine if any differences were obtained between the ligninolytic enzyme concentrations. In addition, the susceptibility of the residual carbohydrates to hydrolysis with a commercially available cellulase was also evaluated.