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Changes in free radical profiles during the callogenesis of responsive and recalcitrant potato genotypes

Published online by Cambridge University Press:  05 December 2011

E. Bailey ,
N. Deighton ,
S. A. Clulow ,
B. A. Goodman  and
E. E. Benson
E. Bailey
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK, and Department of Molecular and Life Sciences, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK
N. Deighton
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
S. A. Clulow
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
B. A. Goodman
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK
E. E. Benson
Affiliation:
Department of Molecular and Life Sciences, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK
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Extract

Changes of the in vitro morphogenetic state may be achieved for some potato genotypes, but others are unresponsive (recalcitrant). Although the biochemical basis for somatic recalcitrance is unknown, evidence suggests that two different aspects of oxidative stress may be involved. Phenolic oxidation is a major problem in manipulating cultures of woody plant species (Thorpe & Harry 1990) and lipid peroxidation has been associated with recalcitrance in monocotyledonous plants (Cutler et al. 1989; Benson et al. 1992). Both oxidative phenomena are believed to be free-radical mediated, but to date there is no reported direct evidence for the formation of free radicals during plant tissue culture callogenesis. The objectives of the present study were twofold; to assess the feasibility of using electron paramagnetic resonance (EPR) spectroscopy to detect free radicals directly in plant tissue cultures and to investigate free radical activity during dedifferentiation of responsive and unresponsive potato genotypes.

Type
Short Communications
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 243 - 246
Copyright
Copyright © Royal Society of Edinburgh 1994

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References

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