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Relation between protein composition and germination capacity of sunflower seeds

Published online by Cambridge University Press:  19 September 2008

C. Reuzeau ,
D. Goffner  and
G. Cavalié
C. Reuzeau*
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
D. Goffner
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
G. Cavalié
Affiliation:
University Paul Sabatier, Plant Physiology Institute, URA CNRS no 1457, 118, route de Narbonne, 31062 Toulouse, France
*
* Correspondence
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Abstract

Protein composition of sunflower seeds (Helianthus annuus L.) was analysed to identify a possible marker for germination capacity and to evaluate seed viability precociously after harvest and after natural ageing during storage. Electrophoresis on 2-D gels was used to compare total soluble and in vivo synthesized proteins in two seed lots showing different germination capacities. Differences were observed to a greater extent in in vivo protein synthesis profiles than in total soluble proteins. Inhibition of [35S]methionine uptake and incorporation in seeds with low germinability was also observed. The precise relationship between protein composition and prediction of germinability could not be determined. Other aspects such as membrane damage, capacity for protein synthesis and seed lot homogeneity in relation to germination are discussed.

Keywords

germinability markergermination capacityin vivo protein synthesismembrane damageproteinseedsunflower
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 4 , December 1992 , pp. 223 - 230
Copyright
Copyright © Cambridge University Press 1992

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