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Ionotropic glutamate receptors of amacrine cells of the mouse retina

Published online by Cambridge University Press:  09 March 2006

OLIVIA N. DUMITRESCU ,
DARIO A. PROTTI ,
SRIPARNA MAJUMDAR ,
HANNS ULRICH ZEILHOFER  and
HEINZ WÄSSLE
OLIVIA N. DUMITRESCU
Affiliation:
Department of Neuroanatomy, Max-Planck-Institute for Brain Research, Frankfurt/Main, Germany
DARIO A. PROTTI
Affiliation:
Department of Physiology, University of Sydney, Sydney, Australia
SRIPARNA MAJUMDAR
Affiliation:
Department of Neuroanatomy, Max-Planck-Institute for Brain Research, Frankfurt/Main, Germany
HANNS ULRICH ZEILHOFER
Affiliation:
Department of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen, Erlangen, Germany
HEINZ WÄSSLE
Affiliation:
Department of Neuroanatomy, Max-Planck-Institute for Brain Research, Frankfurt/Main, Germany
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Abstract

The mammalian retina contains approximately 30 different morphological types of amacrine cells, receiving glutamatergic input from bipolar cells. In this study, we combined electrophysiological and pharmacological techniques in order to study the glutamate receptors expressed by different types of amacrine cells. Whole-cell currents were recorded from amacrine cells in vertical slices of the mouse retina. During the recordings the cells were filled with Lucifer Yellow/Neurobiotin allowing classification as wide-field or narrow-field amacrine cells. Amacrine cell recordings were also carried out in a transgenic mouse line whose glycinergic amacrine cells express enhanced green fluorescent protein (EGFP). Agonist-induced currents were elicited by exogenous application of NMDA, AMPA, and kainate (KA) while holding cells at −75 mV. Using a variety of specific agonists and antagonists (NBQX, AP5, cyclothiazide, GYKI 52466, GYKI 53655, SYM 2081) responses mediated by AMPA, KA, and NMDA receptors could be dissected. All cells (n = 300) showed prominent responses to non-NMDA agonists. Some cells expressed AMPA receptors exclusively and some cells expressed KA receptors exclusively. In the majority of cells both receptor types could be identified. NMDA receptors were observed in about 75% of the wide-field amacrine cells and in less than half of the narrow-field amacrine cells. Our results confirm that different amacrine cell types express distinct sets of ionotropic glutamate receptors, which may be critical in conferring their unique temporal responses to this diverse neuronal class.

Keywords

Mouse retinaAmacrine cellsIonotropic glutamate receptors
Type
Research Article
Information
Visual Neuroscience , Volume 23 , Issue 1 , January 2006 , pp. 79 - 90
Copyright
2006 Cambridge University Press

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