Hostname: page-component-5d59c44645-7l5rh Total loading time: 0 Render date: 2024-02-24T00:43:10.310Z Has data issue: false hasContentIssue false

Is the sperm centrosome to blame for the complex polyploid chromosome patterns observed in cleavage stage embryos from an OAT patient?

Published online by Cambridge University Press:  01 February 2007

K. Chatzimeletiou*
The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London SE1 9RY, UK. School of Biology, University of Leeds, Leeds LS2 9JT, UK.
A. J. Rutherford
Assisted Conception Unit, Clarendon Wing, Leeds General Infirmary, Leeds LS2 9NS, UK.
D. K. Griffin
Dept of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK.
A. H. Handyside
The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London SE1 9RY, UK. School of Biology, University of Leeds, Leeds LS2 9JT, UK.
All correspondence to: Katerina Chatzimeletiou, The London Bridge Fertility, Gynaecology and Genetics Centre, 1 St Thomas Street, London, SE1 9RY, UK. Tel: +44 0207 403 3363. e-mail:


Oligoasthenoteratozoospermia (OAT) is defined by a combined low count < 20 × 106 sperm/ml, poor motility < 50 % forward progression or < 25 % rapid linear progression and abnormal morphology (5–8 % normal using Kruger strict criteria) and has been associated with increased levels of sperm aneuploidy. Here we report on the cytogenetic findings from three ‘spare’ embryos from a couple that were referred for ICSI because of OAT. The embryos were processed for sequential FISH in three hybridization rounds using probes for chromosomes 3, 7, 9, 13, 17, 18, 21, X and Y. Molecular cytogenetic analysis of nine chromosomes revealed that all three embryos were female polyploid. One of them was uniformly tetraploid for all chromosomes tested, while the remaining two embryos showed evidence of abnormal postzygotic segregation of chromosomes, causing the derivative blastomeres to have uneven chromosomal constitution. In one of them in particular, the non-disjoining chromosomes showed preferential segregation to the same pole, rather than randomly moving towards either pole, suggesting an abnormal spindle and causing the derivative blastomeres to have significantly uneven chromosomal constitutions. The possible scenarios leading to polyploidy and chromosomal imbalance through cytokinetic failure and subsequent abnormal centrosomal distribution are outlined.

Research Article
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)


Calogero, A.E., De Palma, A., Grazioso, C., Barone, N., Burrello, N., Palermo, I., Gulisano, A., Pafumi, C. & D'Agata, R. (2001 a). High sperm aneuploidy rate in unselected infertile patients and its relationship with intracytoplasmic sperm injection outcome. Human Reprod. 16, 1433–9.Google Scholar
Calogero, A.E., De Palma, A., Grazioso, C., Barone, N., Romeo, R., Rappazzo, G. & D’Agata, R. (2001 b). Aneuploidy rate in spermatozoa of selected men with abnormal semen parameters. Human Reprod. 16, 1172–9.Google Scholar
Chatzimeletiou, K., Morrison, E.E., Panagiotidis, Y., Prapas, N., Prapas, Y., Rutherford, A.J., Grudzinskas, G. & Handyside, A.H (2005 a). Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics. Reprod. Biomed. Online 11, 697710.Google Scholar
Chatzimeletiou, K., Morrison, E.E., Prapas, N., Prapas, Y. & Handyside, A.H. (2005 b). Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy. Human Reprod. 20, 672–82.Google Scholar
Griffin, D.K. & Finch, K. (2005). The genetic and cytogenetic basis of male infertility. Human Fertility 8, 1926.Google Scholar
In't Veld, P.A., Broekmans, F.J., de France, H.F., Pearson, P.L., Pieters, M.H. & van Kooij, R.J. (1997). Intracytoplasmic sperm injection (ICSI) and chromosomally abnormal spermatozoa. Human Reprod. 12, 752–4.Google Scholar
Pang, M.G., Hoegerman, S.F., Cuticchia, A.J., Moon, S.Y., Doncel, G.F., Acosta, A.A. & Kearns, W.G. (1999). Detection of aneuploidy for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 21, X and Y by fluorescence in-situ hybridization in spermatozoa from nine patients with oligoasthenoteratozoospermia undergoing intracytoplasmic sperm injection. Human Reprod. 14, 1266–73.Google Scholar
Pfeffer, J., Pang, M.G., Hoegerman, S.F., Osgood, C.J., Stacey, M.W., Mayer, J., Oehninger, S. & Kearns, W.G. (1999). Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection. Fertility & Sterility 72, 472–8.Google Scholar
Tempest, H.G. & Griffin, D.K. (2004). The relationship between male infertility and increased levels of sperm disomy. Cytogenet. Genome Res. 107, 8394.Google Scholar
Tesarik, J., Mendoza, C. & Greco, E. (2002). Paternal effects acting during the first cell cycle of human preimplantation development after ICSI. Human Reprod. 17, 184–9.Google Scholar