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Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage

Published online by Cambridge University Press:  10 October 2012

Fernando Henrique Biase ,
Robin Edward Everts ,
Rosane Oliveira ,
Weruska Karyna Freitas Santos-Biase ,
Giovana Krempel Fonseca Merighe ,
Lawrence Charles Smith ,
Lúcia Martelli ,
Harris Lewin  and
Flávio Vieira Meirelles
Fernando Henrique Biase
Affiliation:
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto–Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil. Department of Animal Sciences, University of Illinois at Urbana-Champaign, USA.
Robin Edward Everts
Affiliation:
Department of Animal Sciences, University of Illinois at Urbana-Champaign, USA.
Rosane Oliveira
Affiliation:
Department of Animal Sciences, University of Illinois at Urbana-Champaign, USA.
Weruska Karyna Freitas Santos-Biase
Affiliation:
Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos–Universidade de São Paulo, Pirassununga, São Paulo, Brasil.
Giovana Krempel Fonseca Merighe
Affiliation:
Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos–Universidade de São Paulo, Pirassununga, São Paulo, Brasil.
Lawrence Charles Smith
Affiliation:
Centre de Recherche en Reproducion Animale, Faculté de Médicine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
Lúcia Martelli
Affiliation:
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto–Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil.
Harris Lewin
Affiliation:
Department of Animal Sciences, University of Illinois at Urbana-Champaign, USA.
Flávio Vieira Meirelles*
Affiliation:
Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos–Universidade de São Paulo, Pirassununga, São Paulo, Brasil.
*
All correspondence to: Flávio Meirelles. Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos–Universidade de São Paulo, Pirassununga, São Paulo, Brasil. Tel: +55 19 3565 4112; Fax: +55 19 3565 4117. e-mail: meirellf@usp.br
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Summary

The mRNAs accumulated in oocytes provide support for embryo development until embryo genomic activation. We hypothesized that the maternal mRNA stock present in bovine oocytes is associated with embryo development until the blastocyst stage. To test our hypothesis, we analyzed the transcriptome of the oocyte and correlated the results with the embryo development. Our goal was to identify genes expressed in the oocyte that correlate with its ability to develop to the blastocyst stage. A fraction of oocyte cytoplasm was biopsied using micro-aspiration and stored for further expression analysis. Oocytes were activated chemically, cultured individually and classified according to their capacity to develop in vitro to the blastocyst stage. Microarray analysis was performed on mRNA extracted from the oocyte cytoplasm fractions and correlated with its ability to develop to the blastocyst stage (good quality oocyte) or arrest at the 8–16-cell stage (bad quality oocyte). The expression of 4320 annotated genes was detected in the fractions of cytoplasm that had been collected from oocytes matured in vitro. Gene ontology classification revealed that enriched gene expression of genes was associated with certain biological processes: ‘RNA processing’, ‘translation’ and ‘mRNA metabolic process’. Genes that are important to the molecular functions of ‘RNA binding’ and ‘translation factor activity, RNA binding’ were also enriched in oocytes. We identified 29 genes with differential expression between the two groups of oocytes compared (good versus bad quality). The content of mRNAs expressed in metaphase II oocytes influences the activation of the embryonic genome and enables further develop to the blastocyst stage.

Keywords

Blastocyst developmentDevelopmental competenceOocyte
Type
Research Article
Information
Zygote , Volume 22 , Issue 1 , February 2014 , pp. 69 - 79
Copyright
Copyright © Cambridge University Press 2012 

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