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Comparative analysis of biochemical, hormonal, and mineral compositions of preovulatory and cystic ovarian follicles in buffalo during the non-breeding season

Published online by Cambridge University Press:  15 March 2023

Brijesh Kumar Open the ORCID record for Brijesh Kumar [Opens in a new window] ,
B.L. Kumawat ,
F.A. Khan ,
G.K. Das ,
S.K. Maurya ,
P. Chandra ,
Vandana ,
Jai Singh ,
Vikas Sachan  and
M.H. Jan
...Show all authors
Brijesh Kumar*
Affiliation:
Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India
B.L. Kumawat
Affiliation:
Assistant Professor, Dept. of ARGO, CV &AS, (MAFSU), Prabhani, 431 402, Maharashtra, India
F.A. Khan
Affiliation:
Currently: Theriogenology, Department of Population Medicine, Ontario Veterinary College, University of Guelph, Ontario, Canada
G.K. Das
Affiliation:
Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India
S.K. Maurya
Affiliation:
Deen Dayal Upadhyaya Veterinary and Animal Sciences University (DUVASU), Mathura, Uttar Pradesh, 281001, India
P. Chandra
Affiliation:
Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India
Vandana
Affiliation:
Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India
Jai Singh
Affiliation:
Animal Reproduction Division, ICAR- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India
Vikas Sachan
Affiliation:
Deen Dayal Upadhyaya Veterinary and Animal Sciences University (DUVASU), Mathura, Uttar Pradesh, 281001, India
M.H. Jan
Affiliation:
ICAR – Central Institute for Research on Buffaloes, Sub Campus, Nabha (Punjab) 147 201
K. Narayanan
Affiliation:
ICAR-Indian Veterinary Research Institute, Hebbal, Bengaluru Campus 560024, India
*
Author for correspondence: Brijesh Kumar. Animal Reproduction Division, ICAR Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, 243122, India. Tel: +91 9005711815. Fax: 0581-2231238. E-mail: drbrijeshvet02@gmail.com
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Summary

This study is a comparative analysis of the biochemical, hormonal, and mineral compositions of follicular fluid in preovulatory and cystic follicles of water buffalo (Bubalus bubalis). In total, reproductive tracts from 215 buffalo along with intact ovaries were collected randomly from an abattoir. The incidence of cystic conditions found in this study was 3.72% (8/215), involving the right ovary in 62.5% of instances and the left ovary in 37.5% of instances during the non-breeding season. Follicular fluid was aspirated from preovulatory follicles (12–15 mm diameter, oestrogen-active, follicular phase or stage IV corpus luteum on one of the two ovaries, n = 10) and cystic follicles (at least 20 mm diameter, no corpus luteum on any one of the two ovaries, n = 8). The follicular fluid samples were assayed for biochemical components (uric acid, creatinine, blood urea nitrogen, cholesterol, total protein, glucose, ascorbic acid, and alkaline phosphatase), hormones (progesterone, estradiol, and insulin), and minerals (calcium, magnesium, phosphorus, copper, zinc, and cobalt). Cystic follicles had greater (P < 0.05) concentrations of creatinine, blood urea nitrogen, cholesterol, progesterone, copper, zinc, and cobalt, and lesser (P < 0.05) concentrations of uric acid, glucose, ascorbic acid, estradiol, insulin, calcium, magnesium, and phosphorus compared with preovulatory follicles. These results indicated the marked differences in follicular fluid composition between preovulatory and cystic follicles in buffalo. Some of the changes were indicative of oxidative stress and disturbed steroidogenesis, two important mechanisms shown to be associated with cystic ovarian disease in various species. Further studies are warranted to investigate whether these differences are directly or indirectly involved in the formation of cystic follicles or are mere manifestations of the condition.

Keywords

BiochemicalBuffaloCystic follicleHormonesMineralsNon-breeding season
Type
Research Article
Information
Zygote , Volume 31 , Issue 3 , June 2023 , pp. 246 - 252
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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