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Effects of epigenetic modifier on the developmental competence and quantitative expression of genes in male and female buffalo (Bubalus bubalis) cloned embryos

Published online by Cambridge University Press:  09 January 2023

Anjit Sandhu Open the ORCID record for Anjit Sandhu [Opens in a new window] ,
Sushil K. Mohapatra Open the ORCID record for Sushil K. Mohapatra [Opens in a new window] ,
Manoj K. Singh ,
Suresh K. Singla ,
Manmohan S. Chauhan  and
Radhey S. Manik
Anjit Sandhu*
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India
Sushil K. Mohapatra
Affiliation:
Department of Animal Biotechnology, College of Veterinary Science and AH, Kamdhenu University, Sardarkrushinagar, Gujarat, India
Manoj K. Singh
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India
Suresh K. Singla
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India
Manmohan S. Chauhan
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India
Radhey S. Manik
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India
*
Author for correspondence: Anjit Sandhu. Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132001, Haryana, India. E-mail: anjitsandhu26@gmail.com
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Summary

Adult male and female Murrah buffalo fibroblast cells were used as donors for the production of embryos using handmade cloning. Both donor cells and reconstructed embryos were treated with 50 nM trichostatin-A (TSA) and 7.5 nM 5-aza-2′-deoxycytidine (5-aza-dC). The blastocyst rate of both treated male (40.1% ± 2.05) and female (37.0% ± 0.83) embryos was significantly lower than in untreated control males (49.7% ± 3.80) and females (47.2% ± 2.44) but their apoptotic index was lower (male, control: 5.90 ± 0.48; treated: 4.96 ± 0.31): (female, control: 8.11 ± 0.67; treated: 6.65 ± 0.43) and epigenetic status in terms of global acetylation and methylation of histone was significantly improved. The expression level of hypoxanthine-guanine phosphoribosyltransferase (HPRT) was higher (P < 0.05) and that of PGK, G6PD, OCT 4, IFN-tau and CASPASE3 was significantly lower (P < 0.05) in treated male blastocyst than control and the expression levels of DNMT1, IGF1R and BCL-XL were not significantly different between the two groups. In the female embryos, the relative mRNA abundance of OCT4 was significantly higher (P < 0.05), and that of XIST and CASPASE3 was significantly lower (P < 0.05) in the epigenetic modifier-treated group compared with that of the control group, whereas the expression levels of HPRT, PGK, G6PD, DNMT1, IFN-tau, IGF1R and BCL-XL were not significantly different between the two groups. In both embryos, a similar effect of treatment was observed on genes related to growth and development, but the effect on the expression of X-linked genes varied. These results indicate that not all X-linked genes respond to TSA and 5-aza-dC treatment in the same manner.

Keywords

BlastocystBuffaloEmbryoCloningEpigenetic
Type
Research Article
Information
Zygote , Volume 31 , Issue 2 , April 2023 , pp. 129 - 139
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Copyright
© National Dairy Research Institute, Karnal, India, 2023. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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