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Expression of the cell cycle control protein cdc25 in cleavage stage bovine embryos

Published online by Cambridge University Press:  26 September 2008

J.M. Jones  and
N.L. First
J.M. Jones
Affiliation:
Department of Meat and Animal ScienceUniversity of WisconsinMadison, Wisconsin, USA.
N.L. First*
Affiliation:
Department of Meat and Animal ScienceUniversity of WisconsinMadison, Wisconsin, USA.
*
Dr Neal First, Department of Meat and Animal Science, University of Wisconsin-Madison, Madison, WI 53706, USA. Telephone: (608) 263-4307. Fax: (608) 262-5157.
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Summary

We have examined the synthesis and expression of a homologue of the cell cycle protein cdc25 by early cleavage stage bovine embryos. cdc25 is the protein phosphatase responsible for activating p34cdc2 by dephosphorylating the threonine 14 (Thr 14) and tyrosine 15 (Tyr 15) residues of p34cdc2. Human cdc25 antibody was utilised in western blots immunoprecipitations to examine the presence and synthesis of cdc25 in bovine embryos. cdc25 is present as a 52 kDa non-phosphorylated and a 66 kDa presumably phosphorylated form in bovine 1−, 2−, 4− and 8−cell embryos. However, cdc25 is actively synthesised only in 8-Cell embryos, indicating that the cdc25 present prior to this stage is inherited from the oocyte. In addition, the synthesis of cdc25 was induced in 2-cell embryos in which cleavage was blocked with the DNA synthesis inhibitor aphidicolin.

Keywords

Bovine embryocdc25Transcription
Type
Article
Information
Zygote , Volume 3 , Issue 2 , May 1995 , pp. 133 - 139
Copyright
Copyright © Cambridge University Press 1995

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