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The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development

Published online by Cambridge University Press:  20 September 2017

Matias A. Sirini ,
Juan Mateo Anchordoquy ,
Juan Patricio Anchordoquy ,
Ana M. Pascua ,
Noelia Nikoloff ,
Ana Carranza ,
Alejandro E. Relling  and
Cecilia C. Furnus
Matias A. Sirini
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Juan Mateo Anchordoquy
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Juan Patricio Anchordoquy
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Ana M. Pascua
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Noelia Nikoloff
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Ana Carranza
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
Alejandro E. Relling
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina. Department of Animal Sciences, The Ohio State University, 1680 Madison Ave., Wooster, Ohio 44691, USA.
Cecilia C. Furnus*
Affiliation:
IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina.
*
All correspondence to: Cecilia C. Furnus. IGEVET – Instituto de Genética Veterinaria ‘Ing. Fernando N Dulout’ (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina. Tel.: +54 221 4211799. E-mail: cfurnus@fcv.unlp.edu.ar
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Summary

The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus–oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated. Results demonstrated that acylated ghrelin did not affect oocyte nuclear maturation and cumulus expansion area. However, it induced cumulus cell (CC) death, apoptosis and DNA damage. The damage increased as a function of the concentration employed. Additionally, the percentages of blastocyst yield, hatching and embryo quality decreased with all acylated ghrelin concentrations tested. Our study highlights the importance of acylated ghrelin in bovine reproduction, suggesting that this metabolic hormone could function as a signal that prevents the progress to reproductive processes.

Keywords

Active ghrelinApoptosisCumulus cellsDNA damageIn vitro embryo development
Type
Research Article
Information
Zygote , Volume 25 , Issue 5 , October 2017 , pp. 601 - 611
Copyright
Copyright © Cambridge University Press 2017 

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