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The regulatory role of miR-20a in bovine cumulus cells and its contribution to oocyte maturation

Published online by Cambridge University Press:  23 April 2021

Eryk Andreas ,
Hari Om Pandey ,
Michael Hoelker ,
Dessie Salilew-Wondim ,
Samuel Gebremedhn ,
Karl Schellander  and
Dawit Tesfaye Open the ORCID record for Dawit Tesfaye [Opens in a new window]
Eryk Andreas
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany Robinson Research Institute, The University of Adelaide, 5000Adelaide, SA, Australia
Hari Om Pandey
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Michael Hoelker
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Dessie Salilew-Wondim
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Samuel Gebremedhn
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Colorado State University, 3105 Rampart Rd, Fort Collins, CO80521, USA
Karl Schellander
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Dawit Tesfaye*
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Colorado State University, 3105 Rampart Rd, Fort Collins, CO80521, USA
*
Author for correspondence: Dawit Tesfaye. Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, 3105 Rampart Rd, Fort Collins, CO, USA. Tel: +1 970 491 8391. E-mail: Dawit.Tesfaye@colostate.edu
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Summary

Dynamic changes in microRNAs in oocyte and cumulus cells before and after maturation may explain the spatiotemporal post-transcriptional gene regulation within bovine follicular cells during the oocyte maturation process. miR-20a has been previously shown to regulate proliferation and differentiation as well as progesterone levels in cultured bovine granulosa cells. In the present study, we aimed to demonstrate the function of miR-20a during the bovine oocyte maturation process. Maturation of cumulus–oocyte complexes (COCs) was performed at 39°C in an humidified atmosphere with 5% CO2 in air. The expression of miR-20a was investigated in the cumulus cells and oocytes at 22 h post culture. The functional role of miR-20a was examined by modulating the expression of miR-20a in COCs during in vitro maturation (IVM). We found that the miR-20a expression was increased in cumulus cells but decreased in oocytes after IVM. Overexpression of miR-20a increased the oocyte maturation rate. Even though not statistically significant, miR-20a overexpression during IVM increased progesterone levels in the spent medium. This was further supported by the expression of STAR and CYP11A1 genes in cumulus cells. The phenotypes observed due to overexpression of miR-20a were validated by BMP15 supplementation during IVM and subsequent transfection of BMP15-treated COCs using miR-20a mimic or BMPR2 siRNA. We found that miR-20a mimic or BMPR2 siRNA transfection rescued BMP15-reduced oocyte maturation and progesterone levels. We concluded that miR-20a regulates oocyte maturation by increasing cumulus cell progesterone synthesis by simultaneous suppression of BMPR2 expression.

Keywords

BMPR2miR-20aOocyte maturationProgesterone
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 435 - 444
Copyright
© University of Bonn, 2021. Published by Cambridge University Press

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