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Tolerance of oocyte plasma membrane to electric current changes after fertilisation

Published online by Cambridge University Press:  26 September 2008

Tan Jing-he ,
Liu Zhong-hua ,
Sun Xing-shen  and
He Gui-xin
Tan Jing-he*
Affiliation:
Department of Animal science, Northeat Agricultural University, Harbin, China.
Liu Zhong-hua
Affiliation:
Department of Animal science, Northeat Agricultural University, Harbin, China.
Sun Xing-shen
Affiliation:
Department of Animal science, Northeat Agricultural University, Harbin, China.
He Gui-xin
Affiliation:
Department of Animal science, Northeat Agricultural University, Harbin, China.
*
Dr Jing-he Tan, Department of Animal scince, Northeast Agricultural University, Harbin, china 150030.
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Summary

The tolerance of the oocyte plasma membrane (oolemma) to electrical pulses (TEP) was investigated for oocytes, zygotes, and embryos at the early and late 2-cell stage. The oocyte survival rate after two electrical pulses (1.4KV/cm for 640μs each) was used as an indicator of the TEP of the oolemma. Survival rate of mid-pronuclear zygotes (94.3%±2.3%) was significantly (p < 0.05) higher than that of recently ovulated (2.1%±1.9%) and in vivo aged (25.1%±2.6%) oocytes; survival rate of in vivo aged oocytes was also significantly higher than that of recently ovulated oocytes. Soon after fertilisation, the survival rate of the oocytes markedly increased, up to 94% at the mid-pronuclear stage. Survival ratedropped thereafter. These results suggest that the characteristics of the oocyte plasma membrane (oolemma) change after fertilisation.

Keywords

FertilisationMouseOocytesPlasma membranePules tolerance
Type
Article
Information
Zygote , Volume 4 , Issue 4 , November 1996 , pp. 275 - 278
Copyright
Copyright © Cambridge University Press 1996

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References

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