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Bacterial communities associated with invasive populations of Bactrocera dorsalis (Diptera: Tephritidae) in China

Published online by Cambridge University Press:  31 May 2016

L.J. Liu ,
I. Martinez-Sañudo ,
L. Mazzon ,
C.S. Prabhakar ,
V. Girolami ,
Y.L. Deng ,
Y. Dai  and
Z.H. Li
L.J. Liu
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China
I. Martinez-Sañudo
Affiliation:
Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Università di Padova – Agripolis, Viale dell'Università, Legnaro, Padova, Italy
L. Mazzon
Affiliation:
Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Università di Padova – Agripolis, Viale dell'Università, Legnaro, Padova, Italy
C.S. Prabhakar
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China Department of Entomology, Bihar Agricultural University, Sabour-813210, Bhagalpur, Bihar, India
V. Girolami
Affiliation:
Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Università di Padova – Agripolis, Viale dell'Università, Legnaro, Padova, Italy
Y.L. Deng
Affiliation:
Xishuangbanna Entry-Exit Inspection and Quarantine Bureau, Xishuangbanna, Yunnan, China
Y. Dai
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China
Z.H. Li*
Affiliation:
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China
*
*Author for correspondence Tel: 86 -10-62733000 Fax: 86-10-62733404 E-mail: lizh@cau.edu.cn
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Abstract

The oriental fruit fly Bactrocera dorsalis (Hendel) is a destructive insect pest of a wide range of fruits and vegetables. This pest is an invasive species and is currently distributed in some provinces of China. To recover the symbiotic bacteria of B. dorsalis from different invasion regions in China, we researched the bacterial diversity of this fruit fly among one laboratory colony (Guangdong, China) and 15 wild populations (14 sites in China and one site in Thailand) using DNA-based approaches. The construction of 16S rRNA gene libraries allowed the identification of 24 operational taxonomic units of associated bacteria at the 3% distance level, and these were affiliated with 3 phyla, 5 families, and 13 genera. The higher bacterial diversity was recovered in wild populations compared with the laboratory colony and in samples from early term invasion regions compared with samples from late term invasion regions. Moreover, Klebsiella pneumoniae and Providencia sp. were two of the most frequently recovered bacteria, present in flies collected from three different regions in China where B. dorsalis is invasive. This study for the first time provides a systemic investigation of the symbiotic bacteria of B. dorsalis from different invasion regions in China.

Keywords

Bactrocera dorsalisbacteria diversityinvasion populationlaboratory colonywild population
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 6 , December 2016 , pp. 718 - 728
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Copyright
Copyright © Cambridge University Press 2016 

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