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Cryopreservation of boar semen using straws

Published online by Cambridge University Press:  03 March 2009

Gao Jun-Feng ,
Zheng Xiao-Feng ,
Ge Li-Jun ,
Lu Qing  and
Rui Rong
Gao Jun-Feng
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Zheng Xiao-Feng
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Ge Li-Jun
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Lu Qing
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
Rui Rong*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China
*
*Corresponding author. E-mail: rrui@njau.edu.cn
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Abstract

An optimal protocol for cryopreservation of boar semen was established. First, the boar semen was pre-diluted with ZORLESCO (ZO) solution and pre-equilibrated at room temperature for 1 h. After adding extender I, spermatozoa were equilibrated at 5°C for 1.5 h; then an equal volume of extender II was added and the spermatozoa equilibrated for 2 h. The resulting spermatozoa were loaded into 0.25 ml straws, equilibrated for 10 min at 3 cm above the surface of liquid nitrogen (LN), then promptly submerged into LN. When thawing, straws were incubated in a water bath at 37°C for 30 s. This procedure yielded the highest post-thaw motility of 0.58±0.03 and plasma integrity of 63.2±1.2%, together with a normal acrosome in 51.4±2.6% of spermatozoa. Abnormal spermatozoa after freezing represented only 14.0±3.0%.

Keywords

boarsemencryopreservation
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 3 , December 2008 , pp. 231 - 236
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 264–269

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