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Genome-wide genic SSR marker development for the endangered Dongxiang wild rice (Oryza rufipogon)

Published online by Cambridge University Press:  27 October 2016

Jiankun Xie ,
Meng Zhang ,
Jia Sun  and
Fantao Zhang
Jiankun Xie
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, People's Republic of China
Meng Zhang
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, People's Republic of China
Jia Sun
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, People's Republic of China
Fantao Zhang*
Affiliation:
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, People's Republic of China
*
*Corresponding author. E-mail: zhang84004@163.com
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Abstract

Dongxiang wild rice (Oryza rufipogon, DXWR), one of the species of common wild rice, is regarded as an important genetic resource for the improvement of cultivated rice (Oryza sativa). Molecular markers are reliable tools that can greatly accelerate the breeding process and have been widely used in various species. In the present study, a total of 3681 genic simple sequence repeat (SSR) markers were developed for DXWR based on transcriptome sequencing technology. Additionally, 25 primer pairs were randomly selected and synthesized for the verification. Among them, 18 (72%) primer pairs were successfully amplified in PCR amplification with genomic DNA of DXWR and also had abundant polymorphisms between DXWR and cultivated rice. These novel genic SSR markers will enrich current genomic resources for DXWR, and provide an effective tool for genetic study and molecular marker assisted breeding for this valuable and endangered germplasm.

Keywords

genetic resourcemolecular markerricewild species
Type
Short Communications
Information
Plant Genetic Resources , Volume 15 , Issue 6 , December 2017 , pp. 566 - 569
Copyright
Copyright © NIAB 2016 

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