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Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil

Published online by Cambridge University Press:  08 June 2010

Lucas Ruberto ,
Susana C. Vazquez ,
Romina L. Dias ,
Edgardo A. Hernández ,
Silvia H. Coria ,
Gustavo Levin ,
Alfredo Lo Balbo  and
Walter P. Mac Cormack
Lucas Ruberto
Affiliation:
Instituto Antártico Argentino, Cerrito 1248 (C1010AAZ), Buenos Aires, Argentina Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina
Susana C. Vazquez
Affiliation:
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina
Romina L. Dias
Affiliation:
Consejo de Investigaciones Científicas (CIC), Calle 526 entre 10 y 11 (1900), La Plata, Buenos Aires, Argentina
Edgardo A. Hernández
Affiliation:
Instituto Antártico Argentino, Cerrito 1248 (C1010AAZ), Buenos Aires, Argentina Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina
Silvia H. Coria
Affiliation:
Instituto Antártico Argentino, Cerrito 1248 (C1010AAZ), Buenos Aires, Argentina Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina
Gustavo Levin
Affiliation:
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina
Alfredo Lo Balbo
Affiliation:
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina
Walter P. Mac Cormack*
Affiliation:
Instituto Antártico Argentino, Cerrito 1248 (C1010AAZ), Buenos Aires, Argentina Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos, Junín 956 (C1113AAD), Buenos Aires, Argentina
*
*Corresponding author: wmac@ffyb.uba.ar
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Abstract

Bioaugmentation is a controversial strategy. In this work, the effect of the inoculum size and the absence of natural microflora on the efficiency of hydrocarbon removal were studied. Two levels of inoculum (106 and 109 CFU g-1) were applied to soil microcosms containing sterile (S6 and S9) and non-sterile (NS6 and NS9) oil contaminated Antarctic soil. Community controls (CC) and biostimulated autochthonous microflora (BAM) were also included. Total heterotrophic aerobic (THAB) and hydrocarbon degrading (HDB) bacteria as well as total petroleum hydrocarbons (TPH) were analysed. At day 0, THAB and HDB counts (CFU g-1) showed no differences among CC, BAM and NS6 but significantly higher values were observed in NS9 and S9. At day 60, three different levels of HDB were observed. The lower level was represented by CC (106 CFU g-1), a second group (5 x 107 CFU g-1) was represented by BAM, NS6, NS9 and S6, and the third level was constituted by S9 (1 x 109 CFU g-1). TPH values at day 60 decreased significantly in all systems excluding the controls. NS6, NS9, S6 and S9 were not different from those corresponding to BAM. Results suggest that the bioaugmentation of a chronically diesel fuel-contaminated Antarctic soil is unlikely to be profitable or beneficial.

Keywords

bacterial consortiumbioremediationhydrocarbon contaminationmicrobiology
Type
Biological Sciences
Information
Antarctic Science , Volume 22 , Issue 5 , October 2010 , pp. 463 - 469
Copyright
Copyright © Antarctic Science Ltd 2010

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