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Extracellular Matrix Molecules Involved in Barnacle Shell Mineralization

Published online by Cambridge University Press:  01 February 2011

María S. Fernández ,
Italo Vergara ,
Alejandro Oyarzún ,
José I. Arias ,
Renato Rodríguez ,
Juan P. Wiff ,
Víctor M. Fuenzalida  and
José L. Arias
María S. Fernández
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
Italo Vergara
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
Alejandro Oyarzún
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
José I. Arias
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
Renato Rodríguez
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
Juan P. Wiff
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
Víctor M. Fuenzalida
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
José L. Arias
Affiliation:
Faculty of Veterinary Sciences and Center for Advanced Interdisciplinary Research in Materials, Universidad de Chile, Santiago, Chile.
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Abstract

Austromegabalanus psittacus is a large (normally up to 30 cm high) sessile balanomorph barnacle from the coast of Chile and South Peru. Its hard shell is composed of twelve calcareous side plates, six parietes and six radii, joined in the form of a truncated cone opened at the top. Plates rest on a basal disk firmly cemented to the substratum. Although the crystalline microstructure of barnacle's shell has been studied to some extent, its organic composition and the mechanisms governing the biomineralization of such highly ordered nanocomposite have remained obscure. By using X-ray diffraction, infrared spectrometry, SEM and TEM electron microscopy, histochemistry, immuno-histochemistry and -ultrastructure, biochemistry and a crystallization assay, we have studied the cell-shell interactions, the crystalline microstructure of the inorganic moiety and the localization of particular macromolecules, and tested their influence on crystallization.The mineral of the plates and basal disk was calcite showing a (104) preferential orientation. Plates were not solid but porous. While parietes have longitudinal canals (from the base to the apex), radii have transversal canals arranged parallel to the base. These canals are not in the center of the plates but displaced to the outside of the shell delimiting a thinner solid outer lamina and a thicker inner one. The inner lamina consisted of parallel calcified layers separated by organic sheets. These sheets showed autofluorescence and consisted of chitin surrounded by proteoglycans and other minor proteins, which seems to be responsible for the fluorescent behaviour. These organic sheets were also organized as several concentric rings around the canals. The shell matrix obtained after decalcification, which surrounded the crystals, also contained a loose net of such proteoglycans. Mantle epithelial cells covered the entire surface of the inner side of the inner lamina and extend to the plate canals. While isolated chitin did not promote or alter calcite crystallization, the proteoglycan-rich fraction dramatically modified crystal morphology and size. As we have demonstrated in another model of biomineralization, such as the eggshell, hereby we suggest that these structured polyanionic proteoglycan moieties could also be part of the regulatory mechanisms of the barnacle shell mineralization.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N1.2
Copyright
Copyright © Materials Research Society 2002

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References

1. Lowenstam, H.A. and Weiner, S., On Biomineralization, (Oxford University Press, Oxford, 1989) p. 324.Google Scholar
2. Mann, S., Webb, J. and Williams, R.J.P., Biomineralization, (VCH, Weinheim, 1989) p. 490.Google Scholar
3. Simkiss, K. and Wilbur, K.M., Biomineralization, (Academic Press, San Diego, 1989) p. 337.Google Scholar
4. Heuer, A.H., Fink, D.J., Laraia, V.J., Arias, J.L., Calvert, P.D., Kendall, K., Messing, G.L., Blackwell, J., Rieke, P.C., Thompson, D.H., Wheeler, A.P., Veis, A. and Caplan, A.I., Science 255, 1098 (1992).Google Scholar
5. Aizenberg, J., Tkachenko, A., Weiner, S., Addadi, L. and Hendler, G., Nature 412, 819 (2001).Google Scholar
6. Albeck, S., Weiner, S. and Addadi, L., Chem. Eur. J. 2, 278 (1996).Google Scholar
7. Belcher, A.M., Wu, X.H., Christensen, R.J., Hansma, P.K., Stucky, G.D. and Morse, D.E., Nature 381, 56 (1996).Google Scholar
8. Falini, G., Albeck, S., Weiner, S. and Addadi, L., Science 271, 67 (1996).Google Scholar
9. Thompson, J.B., Paloczi, G.T., Kindt, J.H., Michenfelder, M., Smith, B.L., Stucky, G., Morse, D.E. and Hansma, P. K., Biophys. J. 79, 3307 (2000).Google Scholar
10. Orme, C.A., Noy, A., Wierzbicki, A., McBride, M.T., Grantham, M., Teng, H.H., Dove, P.M. and DeYoreo, J.J., Nature 411, 775 (2001).Google Scholar
11. Nys, Y., Hincke, M.T., Arias, J.L., García-Ruiz, J.M. and Solomon, S.E., Poultry Avian Biol. Rev. 10, 143 (1999).Google Scholar
12. Dauphin, Y., Int. J. Biol. Macromol. 28, 293 (2001).Google Scholar
13. Weiss, I.M., Góhring, W., Fritz, M. and Mann, K., Biochem. Biophys. Res. Comm. 285, 244 (2001).Google Scholar
14. Darwin, C., A monograph on the sub-class cirripedia, (Ray Soc., London, 1854)Google Scholar
15. Bourget, E., Le Natur. Canadien 104, 281 (1977).Google Scholar
16. Lowenstam, H.A., Weiner, S. and Newman, W.A., in Chemistry and Biology of Mineralized Tissues, edited by Slavkin, H. and Price, P. (Excerpta Medica, Amsterdam, 1992) pp. 73–84.Google Scholar
17. Sauren, Y.M.H.F., Mieremet, R.H.P, Groot, C.G., Koerten, H.K. and Scherft, J.P., J. Histochem Cytochem. 39, 331 (1991).Google Scholar
18. Caterson, B., Christner, J.E., Baker, J.R., and Couchman, J.R., Fed. Proc. Fed. Am. Soc. Exp. Biol. 44, 386 (1985).Google Scholar
19. Mehmet, H., Sculder, P., Tang, P.W., Hounsell, F.F. and Caterson, B., Eur. J. Biochem. 157, 385 (1986).Google Scholar
20. Arias, J.I., Jure, C., Wiff, J.P., Fernandez, M.S., Fuenzalida, V. and Arias, J.L., presented at the 2001 MRS Fall Meeting, Boston, MA, 2001 (Mater. Res. Soc. Proc. in press).Google Scholar
21. Fernandez, M.S., Moya, A., Lopez, L. and Arias, J.L., Matrix Biol. 19, 793 (2001).sGoogle Scholar
22. Arias, J.L. and Fernandez, M.S., World's Poultry Sci. J. 57, 349 (2001).Google Scholar
23. Levi-Kalisman, Y., Falini, G., Addadi, L. and Weiner, S., J. Struct. Biol. 135, 8 (2001).Google Scholar