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Assessing Structure-Property Relations of Diseased Tissues Using Nanoindentation and FTIR

Published online by Cambridge University Press:  17 March 2011

Donna M. Ebenstein ,
Joan M. Chapman ,
Cheng Li ,
David Saloner ,
Joseph Rapp  and
Lisa A. Pruitt
Donna M. Ebenstein
Affiliation:
UCB/UCSF Bioengineering Graduate Group, University of California at Berkeley, Berkeley, CA 94720
Joan M. Chapman
Affiliation:
Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720
Cheng Li
Affiliation:
Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720
David Saloner
Affiliation:
UCB/UCSF Bioengineering Graduate Group, University of California at Berkeley, Berkeley, CA 94720 Department of Radiology, University of California at San Francisco, San Francisco, CA 94143
Joseph Rapp
Affiliation:
Department of Vascular Surgery, University of California at San Francisco, San Francisco, CA 94143
Lisa A. Pruitt
Affiliation:
UCB/UCSF Bioengineering Graduate Group, University of California at Berkeley, Berkeley, CA 94720 Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720
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Abstract

Disease processes are often associated with changes in tissue composition. For example, in atherosclerosis lipid and calcification are often found in the artery wall, whereas in healthy arteries the tissue microstructure is dominated by highly organized collagen. Such variations in composition likely result in changes in the material properties of the tissue. However, this relationship has not been fully investigated in atherosclerotic vessels. Using a combination of nanoindentation and spectroscopic techniques, our goal was to assess how changes in tissue composition affect the tissue's mechanical properties. Fourier Transform Infrared Spectroscopy (FTIR) was used to assess the biochemical composition of the tissue samples, such as the lipid and calcium content of fibrous tissues in diseased arteries. Nanoindentation was used to measure the local mechanical properties of the same tissue samples. This information was then correlated by position in the sample to assess the contributions of different constituents to the overall structure-property relations of these tissues.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 711: Symposia FF/GG/HH – Advanced Biomaterials--Characterization, Tissue Engineering and Complexity , 2001 , FF4.2.1
Copyright
Copyright © Materials Research Society 2002

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References

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