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Development and characterization of polymorphic genomic-SSR markers in Asian long-horned beetle (Anoplophora glabripennis)

Published online by Cambridge University Press:  10 April 2017

Zhaoyang Liu ,
Jing Tao  and
Youqing Luo
Zhaoyang Liu
Affiliation:
Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, No.35 Tsinghua East Road, Haidian District, Beijing 100083, People's Republic of China
Jing Tao*
Affiliation:
Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, No.35 Tsinghua East Road, Haidian District, Beijing 100083, People's Republic of China
Youqing Luo*
Affiliation:
Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, No.35 Tsinghua East Road, Haidian District, Beijing 100083, People's Republic of China
*
*Author for correspondence Phone: 86-11-8810588460 Fax: 86-10-62336302 E-mail: taojing1029@hotmail.com, youqingluo@126.com
*Author for correspondence Phone: 86-11-8810588460 Fax: 86-10-62336302 E-mail: taojing1029@hotmail.com, youqingluo@126.com
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Abstract

The Asian long-horned beetle (ALB), Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae: Lamiinae), is a wood-borer and polyphagous xylophage that is native to Asia. It infests and seriously harms healthy trees, and therefore is a cause for considerable environmental concern. The analysis of population genetic structure of ALB and sibling species Anoplophora nobilis (Ganglbauer) will not only help to clarify the relationship between environmental variables and mechanisms of speciation, but also will enhance our understanding of evolutionary processes. However, the known genetic markers, particularly microsatellites, are limited for this species. SSRLocator software was used to analyze the distribution and frequencies of genomic simple sequence repeat (SSR), to infer the basic characteristics of repeat motifs, and to design primers. We developed SSR loci of 2–6 repeated units, including 10,650 perfect SSRs, and found 140 types of repeat motifs. A total of 2621 SSR markers were discovered in ALB whole-genome shotgun sequences. 48 pairs of SSR primers were randomly chosen from 2621 SSR markers, and half of these 48 pairs were polymorphic containing 4 di-, 7 tri-, 2 tetra-, and 11-hexamer SSRs. Four populations test the effectiveness of the primers. These results suggest that our method for whole-genome SSR screening is feasible and efficient, and the SSR markers developed in this study are suitable for further population genetics studies of ALB. Moreover, they may also be useful for the development of SSRs for other Coleoptera.

Keywords

SSRAnoplophora glabripennis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 6 , December 2017 , pp. 749 - 755
Copyright
Copyright © Cambridge University Press 2017 

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