Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-26T13:13:54.210Z Has data issue: false hasContentIssue false
  • English
  • Français

Optical Manipulation of Objects in Microfluidic Devices

Published online by Cambridge University Press:  01 February 2011

Erhan Ata ,
Aaron L. Birkbeck ,
Mihrimah Ozkan ,
Cengiz S. Ozkan ,
Richard Flynn ,
Mark Wang  and
Sadik Esener
Erhan Ata
Affiliation:
Electrical Engineering Department, University of California, San Diego
Aaron L. Birkbeck
Affiliation:
Electrical Engineering Department, University of California, San Diego
Mihrimah Ozkan
Affiliation:
Electrical Engineering Department, University of California, Riverside
Cengiz S. Ozkan
Affiliation:
Mechanical Engineering Department, University of California, Riverside
Richard Flynn
Affiliation:
Electrical Engineering Department, University of California, San Diego
Mark Wang
Affiliation:
Electrical Engineering Department, University of California, San Diego
Sadik Esener
Affiliation:
Electrical Engineering Department, University of California, San Diego
Get access

Abstract

In this paper, we present object manipulation methodologies in microfluidic devices based on object-photon interactions. Devices were fabricated by polydimethylsiloxane (PDMS) elastomer molding of channel structures over photolithographically defined patterns using a thick negative photoresist. Inorganic objects including polystyrene spheres and organic objects including live cells were transferred into fluidic channels using a syringe pump. The objects were trapped and manipulated within the fluidic channels using optical tweezers formed by VCSEL arrays, with only a few mW of optical power. We have also shown that it is possible to manipulate multiple objects as a whole assemble by using an optically-trapped particle as a handle, or an “optical handle”. Optical manipulation will have applications in biomedical devices for drug discovery, cytometry and cell biology research.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 729: Symposium U – MEMS and BioMEMS , 2002 , U1.6
Copyright
Copyright © Materials Research Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1) Paulus, A., “Planar glass and plastic micromachined devices for drug development in the post-genomic era”, Proceedings of International Conference on Microtechnologies: MICRO.tec 2000, Vol.2, pp. 499–504 (2000).Google Scholar
2) Roberts, K.; Williamson, F., Cibuzar, G. and Thomas, L., “The fabrication of an array of microcavities utilizing SU-8 photoresist as an alternative ‘LIGA’ technology”, Proceedings of the Thirteenth Biennial University/Government/Industry Microelectronics Symposium, pp. 139–41 (1999).Google Scholar
3) Richardson, J., Hawkins, P. and Luxton, R., “The use of coated paramagnetic particles as a physical label in a magneto-immunoassay”, Biosensors & Bioelectronics, vol. 16, (no. 9-12), Elsevier (2001).Google Scholar
4) Khandurina, J., “Integrated system for rapid PCR-based DNA analysis in microfluidic devices”, Analytical Chemistry, 72, pp. 2995–3000 (2000).Google Scholar
5) Buccholz, B.A., “Microchannel DNA sequencing matrices with a thermally controlled viscosity switch”, Analytical Chemistry, 73, pp. 157–164 (2001).Google Scholar
6) Xia, Y. and Whitesides, G. M., , Angew., “Soft Lithography”, Chem. Int. Ed. Engl. 1998, 37, 550–575.Google Scholar
7) Chu, D.T., Jeon, N.L., Huang, S., Kane, R., Wargo, C., Choi, I.S., Ingber, D., Whitesides, G., “Patterned deposition of cells and proteins onto surfaces by using three-dimensional systems”, PNAS, 97, 6, pp. 2408–2413 (2000).Google Scholar
8) Sasaki, K., Koshioka, M., Misawa, H., Kitamura, N., and Masuhara, H., “Pattern formation and flow control of fine particles by laser-scanning micromanipulation,” Opt. Lett. 16, 1463–1465 (1991).Google Scholar
9) Dufresne, E. R. and Grier, D. G., “Optical tweezer arrays and optical substrates created with diffractive optics,” Rev. Sci. Instrum. 69, 1974–1977 (1998).Google Scholar
10) Reicherter, M., Haist, T., Wagemann, E. U., and Tiziani, H. J., “Optical particle trapping with computer-generated holograms written on a liquid-crystal display,” Opt. Lett. 24, 608–610 (1999)Google Scholar
11) Kibar, O., Flynn, R. A., and Esener, S. C., OSA Topical Meeting on Spatial Light Modulators Technical Digest (Optical Society of America, Washington DC), 49–51 (1999).Google Scholar