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Nanomonitors: Electrical Immunoassays for Protein Biomarker Profiling

Published online by Cambridge University Press:  01 February 2011

Manish Bothara ,
Ravi K Reddy ,
Thomas Barrett ,
John Carruthers  and
Shalini Prasad
Manish Bothara
Affiliation:
botharam@egmail.com, Portland State University, Electrical and Computer Engineering, 1900 SW Fourth Avenue Bldg,, Wilsonville, OR, 97201, United States
Ravi K Reddy
Affiliation:
ravikiran.k.reddy@gmail.com, Portland State University, Electrical and Computer Engineering, Portland, OR, 97201, United States
Thomas Barrett
Affiliation:
barretth@ohsu.edu, Oregon Health and Science University, Veteran Affairs, Portland, OR, 97201, United States
John Carruthers
Affiliation:
john.carruthers@mindspring.com, Portland State University, Physics Department, Portland, OR, 97201, United States
Shalini Prasad
Affiliation:
sprasad@pdx.edu, Portland State University, Electrical and Computer Engineering, Portland, OR, 97201, United States
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Abstract

The objective of this research is to develop a “point-of-care” device for early disease diagnosis through protein biomarker characterization. Here we present label-free, high sensitivity detection of proteins with the use of electrical immunoassays that we call Nanomonitors. The basis of the detection principle lies in the formation of an electrical double layer and its perturbations caused by proteins trapped in a nanoporous alumina membrane over a microelectrode array platform. High sensitivity and rapid detection of two inflammatory biomarkers, C-reactive protein (CRP) and Myeloperoxidase (MPO) in pure and clinical samples through label-free electrical detection were achieved. The performance metrics achieved by this device makes it suitable as a “lab-on-a-chip” device for protein biomarker profiling and hence early disease diagnosis.

Keywords

biomedicallithography (removal)nanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1106: Symposium PP – The Business of Nanotechnology , 2008 , 1106-PP03-02
Copyright
Copyright © Materials Research Society 2008

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