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Association of distributions of three types of germinal vesicle stage oocytes with the canine follicle location in the ovary

Published online by Cambridge University Press:  21 July 2010

L.X. Wang ,
S. Wang ,
J. Hou ,
R.L. Hu  and
X.R. An
L.X. Wang
Affiliation:
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
S. Wang
Affiliation:
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
J. Hou
Affiliation:
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
R.L. Hu
Affiliation:
Institute of Veterinary Medicine Academy of Military Medical Science of PLA, Changchun 130062, China.
X.R. An*
Affiliation:
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan-ming-yuan West Road, Beijing 100094, China.
*
All correspondence to: X.R An, State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuan-ming-yuan West Road, Beijing 100094, China. Tel: +86 10 627333 5522. Fax: +86 10 62733463. e-mail: wlxue@163.com
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Summary

The objective of current study was to compare the nuclear configurations of canine oocytes recovered from between follicles after isolation. Follicles isolated were classified into follicle-S (follicles located in the ovarian surface) and follicle-I (follicles located inside the ovary) based on the follicle location in the ovary. Nuclear stages of canine oocytes recovered from follicle-S and follicle-I were examined by phase-contrast microscopy after isolation. Results demonstrated that canine GV stage oocytes can be classified into three types based on the status of the nuclear envelope, nucleolus, and chromatin: type A, type B, and type C. In follicle-S group, the majority (95.5%) of canine GV stage oocytes was of type B. All canine GV stage oocytes recovered from follicle-S (including type B and type C) were characterized by nuclear envelope disappearance prior to nucleolus collapse. In contrast, in follicle-I group, the majority (60.2%) of canine GV stage oocytes was of type C. Unexpectedly, a small proportion of canine GV stage oocytes from follicle-I (donated type A) were characterized by nuclear envelope disappearance following nucleolus collapse. In conclusion, nuclear configurations of each type of canine GV stage oocytes may differ from each other. Distributions of each type of canine GV stage oocytes may associate with the follicle location in the ovary.

Keywords

Canine oocytesGV stageNuclear envelopeNucleolusFollicle-S
Type
Research Article
Information
Zygote , Volume 19 , Issue 1 , February 2011 , pp. 91 - 95
Copyright
Copyright © Cambridge University Press 2010

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