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Retinol improves in vitro oocyte nuclear maturation under heat stress in heifers

Published online by Cambridge University Press:  11 July 2012

M.J. Maya-Soriano ,
E. Taberner  and
M. López-Béjar
M.J. Maya-Soriano*
Affiliation:
Department of Animal Health and Anatomy, Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain.
E. Taberner
Affiliation:
Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, Barcelona, Spain.
M. López-Béjar*
Affiliation:
Department of Animal Health and Anatomy, Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain.
*
All corresponding to: M.J. Maya-Soriano or M. López-Béjar. Department of Animal Health and Anatomy, Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain. Tel: +34 93 5814615. Fax: +34 93 5812006. e-mail: mariajose.maya@uab.cat or manel.lopez.bejar@uab.cat
All corresponding to: M.J. Maya-Soriano or M. López-Béjar. Department of Animal Health and Anatomy, Faculty of Veterinary, Universitat Autònoma de Barcelona, Edifici V, Campus UAB, 08193 Barcelona, Spain. Tel: +34 93 5814615. Fax: +34 93 5812006. e-mail: mariajose.maya@uab.cat or manel.lopez.bejar@uab.cat
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Summary

Heat stress (HS) is especially harmful for bovine ovarian follicle development and oocyte competence. Furthermore, HS causes premature aging in oocytes due to high levels of reactive oxygen species (ROS), involved in the harmful effects over the oocyte maturation and the steroidogenic activity of follicular cells. In this study, the presumptive protective effects of antioxidant agents on heat-stressed oocytes were evaluated. Heifer oocytes were matured for 22 h under control (38°C) and HS conditions (41.5°C at 18–21 h of maturation). For each oocyte, nuclear stage and cortical granule (CG) distribution were evaluated. Steroidogenic activity of cumulus cells was also recorded. The antioxidant agents used in the study were: retinol (1.43 μg/ml), retinyl (0.28 μg/ml) and oleic acid (0.05 mg/ml). Based on a chi-squared test (P < 0.05), HS affected negatively the metaphase II (MII) progression and produced a premature CG exocytosis. Retinol improved the oocyte MII progression. However, retinyl and oleic acid, at the concentrations used in this study, could not counteract adverse effects of HS. A decrease in progesterone and increase in estradiol availability were observed when retinyl and oleic acid were supplemented to the maturation medium, respectively. In conclusion, retinol proved to be valuable in heat-stressed oocytes protecting nuclear maturation.

Keywords

Heat stressOleic acidOocyteRetinolRetinyl
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 377 - 384
Copyright
Copyright © Cambridge University Press 2012 

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