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The Use of Electrospun Polycaprolactone as a Dermal Scaffold for Skin Tissue Engineering

Published online by Cambridge University Press:  31 January 2011

Ming Chen ,
Manisha Chopra  and
Sankha Bhowmick
Ming Chen
Affiliation:
g_m1chen@umassd.edu, University of Massachusetts Dartmouth, Biomedical Engineering and Biotechnology, North Dartmouth, Massachusetts, United States
Manisha Chopra
Affiliation:
mchopra@umassd.edu, University of Massachusetts Dartmouth, Biomedical Engineering and Biotechnology, North dartmouth, Massachusetts, United States
Sankha Bhowmick
Affiliation:
sbhowmick@umassd.edu, University of Massachusetts Dartmouth, Mechanical Engineering, North Dartmouth, Massachusetts, United States
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Abstract

Rapid healing of acute and chronic skin defects is an important objective. In the present work, we report on the design and feasibility of a co-culture system for fibroblasts and keratinocytes by using electrospun polycaprolactone (PCL) scaffolds. Specifically, we quantified the effect of scaffold fiber diameter on keratinocyte attachment, proliferation and differentiation along with collagen secretion by fibroblasts post vacuum seeding with fibroblasts at various depths. The results show that fibroblasts secrete more collagen and keratinocytes differentiate more on 400 nm scaffolds than on 1000 nm scaffolds. Also, fibroblasts co-cultured with keratinocytes provide increased collagen secretion and keratinocyte differentiation. These results suggest that the fiber architecture can be a useful parameter in skin tissue engineering.

Keywords

nanostructurebiomaterialpolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1235: Symposium RR – Engineering Biomaterials for Regenerative Medicine , 2009 , 1235-RR05-04
Copyright
Copyright © Materials Research Society 2010

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