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Blockade of amiloride-sensitive sodium channels alters multiple components of the mammalian electroretinogram

Published online by Cambridge University Press:  02 June 2005

LAURA M. BROCKWAY ,
DALE J. BENOS ,
KENT T. KEYSER  and
TIMOTHY W. KRAFT
LAURA M. BROCKWAY
Affiliation:
Vision Science Research Center, University of Alabama at Birmingham, Birmingham
DALE J. BENOS
Affiliation:
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham
KENT T. KEYSER
Affiliation:
Vision Science Research Center, University of Alabama at Birmingham, Birmingham
TIMOTHY W. KRAFT
Affiliation:
Vision Science Research Center, University of Alabama at Birmingham, Birmingham Department of Physiological Optics, University of Alabama at Birmingham, Birmingham
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Abstract

Retinal neurons and Müller cells express amiloride-sensitive Na+ channels (ASSCs). Although all major subunits of these channels are expressed, their physiological role is relatively unknown in this system. In the present study, we used the electroretinogram (ERG) recorded from anesthetized rabbits and isolated rat and rabbit retina preparations to investigate the physiological significance of ASSCs in the retina. Based upon our previous study showing expression of α-ENaC and functional amiloride-sensitive currents in rabbit Müller cells, we expected changes in Müller cell components of the ERG. However, we observed changes in other components of the ERG as well. The presence of amiloride elicited changes in all major components of the ERG; the a-wave, b-wave, and d-wave (off response) were enhanced, while there was a reduction in the amplitude of the Müller cell response (slow PIII). These results suggest that ASSCs play an important role in retinal function including neuronal and Müller cell physiology.

Keywords

RetinaMüller cellSlow PIIIEpithelial sodium channelAcid-sensing ion channel
Type
Research Article
Information
Visual Neuroscience , Volume 22 , Issue 2 , March 2005 , pp. 143 - 151
Copyright
© 2005 Cambridge University Press

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