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The Development of Silicon Carbide Based Electrode Devices for Central Nervous System Biomedical Implants

Published online by Cambridge University Press:  31 January 2011

Christopher Frewin ,
Alexandra Oliveros ,
Christopher Locke ,
Irina Filonova ,
Justin Rogers ,
Edwin Weeber  and
Stephen E. Saddow
Christopher Frewin
Affiliation:
clfrewin@mail.usf.eduhlodyn676@msn.com, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Alexandra Oliveros
Affiliation:
amolive4@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Christopher Locke
Affiliation:
clocke@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Irina Filonova
Affiliation:
amerishka@gmail.com, University of South Florida, Molecular Pharmacology and Physiology Department, Tampa, Florida, United States
Justin Rogers
Affiliation:
jrogers3@health.usf.edu, University of South Florida, Molecular Pharmacology and Physiology Department, Tampa, Florida, United States
Edwin Weeber
Affiliation:
eweeber@health.usf.edu, University of South Florida, Florida Center of Excellence for Biomolecular Identification and Targeted Therapeutics (FCoE-BITT), Tampa, Florida, United States
Stephen E. Saddow
Affiliation:
saddow@ieee.org, University of South Florida, Electrical Engineering, Tampa, Florida, United States
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Abstract

Brain machine interface (BMI) technology has been demonstrated to be a therapeutic solution for assisting people suffering from damage to the central nervous system (CNS), but BMI devices using implantable neural prosthetics have experienced difficulties in that they are recognized by glial cells as being foreign material, which leads to an immune response cascade process called gliosis. One material, cubic silicon carbide (3C-SiC), may provide an excellent solution for the generation of an implantable neural prosthetic interface component of a BMI system. We have recently reported on the biocompatibility of 3C-SiC with immortalized cells, and have extended this work by demonstrating neural cell action potential instigation via an electrode type device. Biocompatibility assessment of 3C-SiC was accomplished using in vitro methodology. 96 hour MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays were performed to determine neural cell viability. Atomic force microscopy (AFM) was used to quantify attached cell morphology and determine lamellipodia/ filopodia interaction with the surface of the semiconductor. It was seen that neurons show excellent viability, cell morphology, and good lamellipodia/ filopodia permissiveness when interacting with 3C-SiC. A neuronal activation device (NAD), based on the planar Michigan microelectrode probe, was constructed from 3C-SiC with the goal of activating an action potential within a neuron. In order to illicit an action potential, neurons were seeded on the NAD device and then they were subjected to a biphasic square pulse signal. Successful action potential activation was recorded through the use of Rhod-2, a Ca2+ sensitive fluorescent dye. Based on these results, 3C-SiC may be an excellent material platform for neural prosthetics.

Keywords

biomaterialbiomedicaldevices
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1236: Symposium SS – Biosurfaces and Biointerfaces , 2009 , 1236-SS01-02
Copyright
Copyright © Materials Research Society 2010

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References

[1] Lebedev, M. A. and Nicolelis, M. A. L. “Brain-machine interfaces: past, present and future”, Trends Neurosci., vol. 29, no. 9, pp. 536–546, 2006.Google Scholar
[2] Sanchez, J. C. Mahmoudic, B. c, J. DiGiovanna, and Principe, J. C. “Exploiting coadaptation for the design of symbiotic neuroprosthetic assistants”, Neural Networks, vol. 22, pp. 305–315, 2009.Google Scholar
[3] Wise, K. D. Sodagar, A. M. Yao, Y. Gulari, M. N. Perlin, G. E. and Najafi, K. “Microelectrodes, microelectronics, and implantable neural microsystems”, Proceedings of the IEEE, vol. 96, no. 7, pp. 1184–1202, 2008.Google Scholar
[4] Polikov, V. S. Tresco, P. A. and Reichert, W. M. “Response of brain tissue to chronically implanted neural electrodes”, J. of Neuroscience Methods, vol. 148, pp. 1–18, August 2005.Google Scholar
[5] Locke, C. Kravchenko, G. Waters, P. Reddy, J. Deva, Volinsky, A. A. Frewin, C. L. and Saddow, S. E. “3C-SiC films on Si for MEMS applications: Mechanical Properties,” Materials Science Forum, vol. 615-617, pp. 633–636, 2009.Google Scholar
[6] Kordina, O. and Saddow, S. E.. “Silicon carbide overview” in Advances in Silicon Carbide Processing and Applications, Saddow, S. E. and Agarwal, A. Eds., Boston, MA, USA:Artech House, Inc., 2004, pp. 2–3, 7-8, 18.Google Scholar
[7] Frewin, C. L. Jaroszeski, M. Weeber, E. Muffly, K. E. Kumar, A. Peters, M. Oliveros, A. and Saddow, S.E. “Atomic Force Microscopy Analysis of Central Nervous System Cell Morphology on Silicon Carbide and Diamond Substrates,” Journal of Molecular Recognition, vol. 22, pp. 380–388, (2009).Google Scholar
[8] Reddel, R. R. “The role of senescence and immortalization in carcinogenesis”, Carcinogenesis, vol. 21, no. 3, pp. 477–484, 2000.Google Scholar
[9] Severino, A. Frewin, C. Anzalone, R. Bongiorno, C. Fiorenza, P. D'Arrigo, G., Giannazzo, F., Foti, G. Via, F. La and Saddow, S. E. “Growth of 3C-SiC on Si: Influence of Process Pressure,” Materials Science Forum Vols. 600-603 (2009) pp 211–214.Google Scholar
[10] Huan, C. M. Kao, L. S.., “Nerve growth factor, epidermal growth factor, and insulin differentially potentiate ATP-induced [Ca2+] rise and dopamine secretion in PC12 cells:”, J Neurochem, vol. 66, pp. 124–130, 1996.Google Scholar
[11] [34] Hamill, O. P. Marty, A. Neher, E. Sakmann, B. Sigworth, F. J. “Improved patchclamp techniques for high-resolution current recording from cells and cell-free membrane patches”, Pflugers Arch, vol. 391, pp. 85–100, 1981.Google Scholar