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The effect of mealybug Pseudococcus longispinus (Targioni Tozzetti) infestation of different density on physiological responses of Phalaenopsis × hybridum ‘Innocence’

Published online by Cambridge University Press:  01 April 2015

I. Kot ,
K. Kmieć ,
E. Górska-Drabik ,
K. Golan ,
K. Rubinowska  and
B. Łagowska
I. Kot*
Affiliation:
Department of Entomology, University of Life Sciences in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin, Poland
K. Kmieć
Affiliation:
Department of Entomology, University of Life Sciences in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin, Poland
E. Górska-Drabik
Affiliation:
Department of Entomology, University of Life Sciences in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin, Poland
K. Golan
Affiliation:
Department of Entomology, University of Life Sciences in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin, Poland
K. Rubinowska
Affiliation:
Department of Plant Physiology, University of Life Sciences in Lublin, ul. Akademicka 15, 20-950 Lublin, Poland
B. Łagowska
Affiliation:
Department of Entomology, University of Life Sciences in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin, Poland
*
*Author for correspondence Phone: +48815248102 Fax: +48815248103 E-mail: izabela.kot@up.lublin.pl
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Abstract

Cultivated orchids are the most abundantly attacked by polyphagous mealybugs. This study documented how different density of mealybug Pseudococcus longispinus (Targioni Tozzetti) infestation is associated with a response of antioxidative systems of Phalaenopsis × hybridum ‘Innocence’. The degree of cell damage, estimated by electrolyte leakage measurement and the level of thiobarbituric acid reactive substances (TBARS), the content of pigments as well as the activity of antioxidative enzymes and proline level, as measurements of stress and stress compensation in moth orchid were examined. The highest electrolyte leakage (EL) value among samples from colonized plants was found in the orchids from series III (50 individuals/plant), whereas the lowest in the plants from series II (20 individuals/plant). The TBARS content reached the highest level at the lowest number of feeding insects (series I). Peroxidase activity toward guaiacol was significantly increased in series I (5 individuals/plant). The highest catalase activity was recorded in plants colonized by the highest number of scale insects (series III). Whereas, the highest value of proline was in series II. The content of individual photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) in plant tissues did not vary significantly between control and colonized orchids. The results have not confirmed hypothesis that the increasing number of mealybugs occurring on plant enhanced plant physiological response. The degree of longtailed mealybug infestation on plants was positively correlated only with electrolyte leakage and catalase activity in leaf tissues.

Keywords

longtailed mealybugTBARSelectrolyte leakageantioxidantsphotosynthetic pigments
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 3 , June 2015 , pp. 373 - 380
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Copyright
Copyright © Cambridge University Press 2015 

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