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Direct immobilization of DNA on partially functionalized diamond surface

Published online by Cambridge University Press:  01 February 2011

Jung-Hoon Yang ,
Kwang-Soup Song ,
Shouma Kuga  and
Hiroshi Kawarada
Jung-Hoon Yang
Affiliation:
junghoon_yang@moegi.waseda.jp, waseda university, Electronical Engineering and Bioscience, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan, 81-3-5286-3391, 81-3-5286-3391
Kwang-Soup Song
Affiliation:
song@kaw.comm.waseda.ac.jp, Waseda university, Electronical Engineering and Bioscience, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan
Shouma Kuga
Affiliation:
shouma.kuga@gmail.com, Waseda university, Electronical Engineering and Bioscience, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan
Hiroshi Kawarada
Affiliation:
kawarada@waseda.jp, Waseda university, Electronical Engineering and Bioscience, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan
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Abstract

Amino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropatterned diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

Keywords

diamondbiologicalsurface reaction
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D04-51
Copyright
Copyright © Materials Research Society 2007

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References

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