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Labeling Cells with Silver/Dendrimer Nanocomposites

Published online by Cambridge University Press:  01 February 2011

Wojciech Lesniak ,
Xiangyang Shi ,
Anna Bielinska ,
Katarzyna Janczak ,
Kai Sun ,
James R. Baker Jr  and
Lajos P. Balogh
Wojciech Lesniak
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109
Xiangyang Shi
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109
Anna Bielinska
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109
Katarzyna Janczak
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109
Kai Sun
Affiliation:
Electron Microbeam Analysis Laboratory, Ann Arbor, MI 48109
James R. Baker Jr
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109
Lajos P. Balogh
Affiliation:
Center for Biologic Nanotechnology, Ann Arbor, MI 48109 Department of Biomedical Engineering, Ann Arbor, MI 48109 Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109
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Abstract

We have developed water-soluble, biocompatible, fluorescent, and photostable silver/dendrimer nanocomposites that have a potential to be used for in vitro cell labeling. A PAMAM_E5.NH2 dendrimer was used as a template to prepare first a silver-dendrimer complex in an aqueous solution at biologic pH=7.4. Conversion into nanocomposite was achieved by irradiating the solution of the [(Ag+)25-PAMAM_E5.NH2] complex by UV light to reduce the bound Ag+ to zero-valent Ag0 atoms, which were simultaneously trapped in the dendrimer network. Results indicate that the {(Ag0)25-PAMAM_E5.NH2} silver/dendrimer nanocomposite forms positively charged single particles of 4–5 nm under the experimental conditions used. The dendrimer nanocomposite proved to be fluorescent. Toxicity testing of {(Ag0)25-PAMAM_E5.NH2} nanocomposite revealed a behavior similar to the template dendrimer. Intracellular internalization of the silver nanocomposite and cell labeling capabilities was confirmed by confocal microscopy.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.37
Copyright
Copyright © Materials Research Society 2005

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References

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