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Changes in facial nerve function, morphology and neurotrophic factor expression in response to three types of nerve injury

Published online by Cambridge University Press:  25 November 2009

L Zhang ,
Z Fan ,
Y Han ,
L Xu ,
J Luo ,
J Li  and
H Wang
L Zhang
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
Z Fan
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
Y Han
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
L Xu
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
J Luo
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
J Li
Affiliation:
Institute of Eye and ENT, Shandong Clinic Research Institute, Jinan, People's Republic of China
H Wang*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China Institute of Eye and ENT, Shandong Clinic Research Institute, Jinan, People's Republic of China
*
Address for correspondence: Dr Haibo Wang, Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Affiliated to Shandong University, 324 Jingwu Street, Jinan, 250021, P. R. China Fax: 86 531 85187584 E-mail: wanghb7585@hotmail.com
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Abstract

Objective:

To study the changes in facial nerve function, morphology and neurotrophic factor 3 expression in response to three types of nerve injury.

Materials and methods:

Changes in facial nerve function (in terms of blink reflex, vibrissae movement and position of nasal tip) were assessed in 45 rats in response to three types of nerve injury: partial section of the extratemporal segment (group one); partial section of the facial canal segment (group two); and complete transection of the facial canal segment (group three). All facial nerves were then dissected out from the sacrificed animals, on the first, seventh or 21st post-operative day, and the injured segment bisected for subsequent analysis. Morphological change and neurotrophic factor 3 expression in these facial nerve segments were evaluated by means of improved trichrome staining and immunohistochemical analysis, respectively.

Results:

Facial nerve function was more severely damaged and recovery was slower in group two compared with group one, although the facial nerve injury had been identical at the two sites involved. In group three (complete facial canal transection), scattered degenerative fibres were observed at the transection site on the seventh post-operative day. The number of degenerated fibres, assessed on the seventh post-operative day, was markedly increased in group two compared with group one. On the seventh post-operative day, axonal expression of neurotrophic factor 3 was strongly positive, whereas expression in Schwann cells was only weakly positive.

Conclusions:

The facial palsy of the group two rats was more severe than that of group one rats, although their facial nerve function had partly recovered on the 21st post-operative day. Fibre degeneration was not only dispersed throughout the injury site but also occurred throughout the length of the nerve. Neurotrophic factor 3 immunoreactivity increased in facial nerve fibres after partial transection.

Keywords

Facial NerveFacial ParalysisTemporal BoneFractureNerve Degeneration
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 3 , March 2010 , pp. 265 - 271
Copyright
Copyright © JLO (1984) Limited 2009

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References

1Adegbite, AB, Khan, MI, Tan, L. Predicting recovery of facial nerve function following injury from a basilar skull fracture. J Neurosurg 1991;75:759–62CrossRefGoogle ScholarPubMed
2Brodie, HA, Thompson, TC. Management of complications from 820 temporal bone fractures. Am J Otol 1997;18:188–97Google ScholarPubMed
3Melvin, TA, Limb, CJ. Overview of facial paralysis: current concepts. Facial Plast Surg 2008;24:155–63CrossRefGoogle ScholarPubMed
4Chang, CY, Cass, P. Management of facial nerve injury due to temporal bone trauma. Am J Otol 1999;20:96–114Google ScholarPubMed
5McKennan, KX, Chole, RA. Facial paralysis in temporal bone trauma. Am J Otol 1992;13:167–72Google ScholarPubMed
6Binns, PM. Experimental studies of the facial nerve. Trans Am Acad Ophthalmol Otolaryngol 1967;71:665–72Google ScholarPubMed
7Boyle, WF. Evaluation of facial nerve decompression in experimental facial nerve paralysis in monkeys. Laryngoscope 1967;77:1168–78CrossRefGoogle ScholarPubMed
8Boyle, WF. Experimental facial nerve paralysis. An evaluation of surgical and medical therapy. Arch Otolaryngol 1972;95:313–16CrossRefGoogle ScholarPubMed
9Greer, JA, Cody, DT, Lambert, EH, Weiland, LH. Experimental facial nerve paralysis: influence of decompression. Ann Otol Rhinol Laryngol 1974;83:582–95CrossRefGoogle ScholarPubMed
10Darrouzet, V, de Bonfils-Dindart, C, Bébéar, JP. Management of post-traumatic facial paralysis. A decision based on a series of 85 cases [in French]. Neurochirurgie 1998;44:235–46Google ScholarPubMed
11Takeda, T, Takeuchi, S, Kishimoto, S, Saito, H. Interstitial fluid pressure in the facial nerve: relationship between facial nerve pressure and cerebrospinal fluid pressure. Am J Otolaryngol 1989;10:345–50CrossRefGoogle ScholarPubMed
12Böhmer, A, Herzog, J, Dillier, N. Endoneurial fluid pressure in the facial nerve of guinea pigs and rabbits. Am J Otol 1990;11:164–8Google Scholar
13Guerrissi, JO. Facial nerve paralysis after intratemporal and extratemporal blunt trauma. J Craniofac Surg 1997;8:431–7CrossRefGoogle ScholarPubMed
14May, M. Total facial nerve exploration: transmastoid, extralabyrinthine, and subtemporal indications and results. Laryngoscope 1979;89:906–17CrossRefGoogle Scholar
15Nakamura, H, Takada, S, Shimabuku, R, Matsuo, M, Matsuo, T, Negishi, H. Auditory nerve and brainstem responses in newborn infants with hyperbilirubinemia. Pediatrics 1985;75:703–8CrossRefGoogle ScholarPubMed
16Xu, B, Michalski, B, Racine, RJ, Fahnestock, M. Continuous infusion of neurotrophin-3 triggers sprouting, decreases the levels of TrkA and TrkC, and inhibits epileptogenesis and activity-dependent axonal growth in adult rats. Neuroscience 2002;115:1295–308CrossRefGoogle ScholarPubMed