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SERS Biotags (SBTs) for the Quantitative Ratiometric Discrimination between Noncancerous and Cancerous Prostate Cells

Published online by Cambridge University Press:  03 October 2012

Alessia Pallaoro ,
Gary Braun  and
Martin Moskovits
Alessia Pallaoro
Affiliation:
Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA, United States.
Gary Braun
Affiliation:
Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA, United States.
Martin Moskovits
Affiliation:
Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA, United States.
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Abstract

We report on a multiplexed, ratiometric method that can confidently distinguish between cancerous and non-cancerous epithelial prostate cells in vitro, as demonstrated by a double blind experiment. The technique is based on bright SERRS biotags (SBTs) infused with unique Raman reporter molecules, and carrying cell-specific peptides. Two sets of SERRS biotags were used. One targets the neuropilin-1 (NRP) receptors of cancer cells through the RPARPAR peptide. The other functions as a positive control (PC) and binds to both non-cancerous and cancer cells through the HIV derived TAT peptide. Averaging the SERRS signal over a given cell yielded an NRP/PC ratio from which a robust quantitative measure of the overexpression of the NRP-1 by the cancer cell line was extracted. The use of a local, on-cell reference produces quantitative, statistically robust measures of overexpression independent of such sources of uncertainty such as variations in the location of the focal plane, the local cell concentration and turbidity.

Keywords

Raman spectroscopycluster assemblybiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1468: Symposium VV – Nanomedicine for Molecular Imaging and Therapy , 2012 , mrss12-1468-vv04-03
Copyright
Copyright © Materials Research Society 2012

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References

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