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Long term dietary supplementation with microalgae increases plasma docosahexaenoic acid in milk and plasma but does not affect plasma 13,14-dihydro-15-keto PGF2α concentration in dairy cows

Published online by Cambridge University Press:  12 February 2020

Bethan E. Till ,
James A. Huntington ,
Kirsty E. Kliem ,
Jules Taylor-Pickard  and
Liam A. Sinclair
Bethan E. Till
Affiliation:
Department of Animal Production, Welfare and Veterinary Sciences, Harper Adams University, Newport, ShropshireTF10 8NB, UK
James A. Huntington
Affiliation:
Department of Animal Production, Welfare and Veterinary Sciences, Harper Adams University, Newport, ShropshireTF10 8NB, UK
Kirsty E. Kliem
Affiliation:
Department of Animal Sciences, School of Agriculture, Policy and Development, University of Reading, ReadingRG6 6AR, UK
Jules Taylor-Pickard
Affiliation:
Alltech Biotechnology Centre, Summerhill Road, Dunboyne, Ireland
Liam A. Sinclair*
Affiliation:
Department of Animal Production, Welfare and Veterinary Sciences, Harper Adams University, Newport, ShropshireTF10 8NB, UK
*
Author for correspondence: Liam Sinclair, Email: lsinclair@harper-adams.ac.uk
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Abstract

The aims of the study were to determine the long-term effects of dietary supplementation with microalgae (SCIM) on milk and blood fatty acid (FA) composition and reproductive hormones in early lactation dairy cows. Sixty Holstein–Friesian dairy cows (30 per treatment) were unsupplemented (Control) or supplemented with 100 g of SCIM (Schizochytrium limacinum sp.) per cow per day from 25 ± 0.5 d post-partum for 98 d. Intake and milk yield were recorded daily, with milk samples collected at weeks 0, 1, 2, 4, 8 and 14, and blood samples collected from 12 representative pairs per treatment at weeks 0, 2, 4, 8, and 14 for subsequent analysis of FA, β-hydroxybutyrate, non-esterified fatty acids and glucose. At 33 ± 0.9 d postpartum the oestrus cycle of 24 cows (12 per treatment) were synchronized and plasma 13,14-dihydro-15-keto PGF2α (PGFM) concentrations determined following an oxytocin challenge. Data were analysed by repeated measures analysis of variance. There was no effect of treatment on dry matter intake, milk yield or milk fat content, with mean values across treatments of 22.1 and 40.6, and 37.2 g/kg respectively. Milk fat concentration of C22:6 n-3 increased rapidly in cows receiving SCIM, reaching a maximum of 0.38 g/100 g FA by week 14. Similarly, blood concentration of C22:6 n-3 increased to 1.6 g/100 g FA by week 14 in cows fed SCIM. There was no effect of treatment on plasma metabolites, but plasma glucose was lower in cows fed SCIM compared to the Control at week 2, and higher in week 8. There was no effect of treatment on peak plasma PGFM concentration or area under the curve. It is concluded that feeding SCIM rapidly increases blood and milk concentrations of C22:6 n-3 which are maintained over time, but does not improve plasma PGFM in dairy cows.

Keywords

Dairy cowfatty acidshormonesmilk qualitymicroalgae
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 14 - 22
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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References

Association of Official Analytical Chemists (2012) Official Methods of Analysis, 19th Edn. Washington, DC, USA: Association of Official Analytical Chemists.Google Scholar
Bauman, DE and Griinari, JM (2003) Nutritional regulation of milk fat synthesis. Annual Review of Nutrition 23, 203–227.Google ScholarPubMed
Bichi, E, Hervas, G, Toral, PG, Loor, JJ and Frutos, P (2013) Milk fat depression induced by dietary marine algae in dairy ewes: persistency of milk fatty acid composition and animal performance responses. Journal of Dairy Science 96, 524–532.Google ScholarPubMed
Chilliard, Y, Ferlay, A and Doreau, M (2001) Effect of different types of forages, animal fat or marine oils in cow's diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livestock Production Science 70, 31–48.Google Scholar
Cozzi, G, Ravarotto, L, Gottardo, F, Stefani, AL, Contiero, B, Moro, L, Brscic, M and Dalvit, P (2011) Short communication: reference values for blood parameters in Holstein dairy cows: effects of parity, stage of lactation, and season of production. Journal of Dairy Science 94, 3895–3901.Google Scholar
Dirandeh, E, Towhidi, A, Zeinoaldini, S, Ganjkhanlou, M, Pirsaraei, ZA and Fouladi-Nashta, A (2013) Effects of different polyunsaturated fatty acid supplementations during the postpartum periods of early lactating dairy cows on milk yield, metabolic responses, and reproductive performances. Journal of Animal Science 91, 713–721.Google ScholarPubMed
Ferguson, JD, Galligan, DT and Thomsen, N (1994) Principal descriptors of body condition score in Holstein cows. Journal of Dairy Science 77, 2695–2703.Google ScholarPubMed
Franklin, ST, Martin, KR, Baer, JR, Schingoethe, DJ and Hippen, AR (1999) Dietary marine algae (Schizochytrium sp.) increases concentrations of conjugated linoleic, docosahexaenoic and trans vaccenic acids in milk of dairy cows. Journal of Nutrition 11, 2048–2054.Google Scholar
Gulliver, CE, Friend, MA, King, BJ and Clayton, EH (2012) The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Animal Reproduction Science 131, 9–22.Google ScholarPubMed
Hyeon, KD, Jun, LH, Sardar, AM, Adegbola, AT and Sam Churl, K (2016) Effects of dietary n-6/n-3 fatty acid ratio on nutrient digestibility and blood metabolites of Hanwoo heifers. Animal Science Journal 87, 46–53.Google Scholar
Lock, AL, Tyburczy, C, Dwyer, DA, Harvatine, KJ, Destaillats, F, Mouloungui, Z, Candy, L and Bauman, DE (2007) Trans-10 octadecenoic acid does not reduce milk fat synthesis in dairy cows. The Journal of Nutrition 137, 71–76.Google Scholar
Marques, JA, Del Valle, TA, Ghizzi, LG, Zilio, EMC, Gheller, LS, Nunes, AT, Silva, TBP, Dias, MSS and Grigoletto, NTS, Koontz, AF, da Silva, GG and Rennó, FP 2019 Increasing dietary levels of docosahexaenoic acid-rich microalgae: ruminal fermentation, animal performance and milk fatty acid profile of mid-lactating dairy cows. Journal of Dairy Science 102, 5054–5065.Google ScholarPubMed
Mattos, R, Staples, CR, Arteche, A, Wiltbank, MC, Diaz, FJ, Jenkins, TC and Thatcher, WW (2004) The effects of feeding FO on uterine secretions of PGF2α, milk composition, and metabolic status of periparturient Holstein cows. Journal of Dairy Science 87, 921–932.Google ScholarPubMed
McArt, JAA, Nydam, DV, Oetzel, GR, Overton, TR and Ospina, PA (2013) Elevated non-esterified fatty acids and β-hydroxybutyrate and their association with transition dairy cow performance. The Veterinary Journal 198, 560–570.Google ScholarPubMed
Otto, JR, Freeman, MJ, Malau-Aduli, BS, Nichols, PD, Lane, PA and Malau-Aduli, AEO (2014) Reproduction and fertility parameters of dairy cows supplemented with omega-3 fatty acid-rich canola oil. Annual Research and Review in Biology 10, 1611–1636.Google Scholar
Petit, HV, Dewhurst, RJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungi, H and Mann, GE (2002) Milk production and composition, ovarian function and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85, 889–899.Google ScholarPubMed
Ribeiro, ES, Greco, LF, Bisinotto, RS, Lima, FS, Thatcher, WW and Santos, JEP (2016) Biology of preimplantation conceptus at the onset of elongation in dairy cows. Biology of Reproduction 94(97), 1–18.Google ScholarPubMed
Robinson, RS, Pushpakumara, PG, Cheng, Z, Peters, AR, Abayasekara, DR and Wathes, DC (2002) Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction 124, 119–131.Google ScholarPubMed
Rodney, RM, Celi, P, Scott, W, Breinhild, K and Lean, IJ (2015) Effects of dietary fat on fertility of dairy cattle: a meta-analysis and meta-regression. Journal of Dairy Science 98, 5601–5620.Google ScholarPubMed
Rodriguez-Herrera, M, Khatri, Y, Marsh, SP, Posri, W and Sinclair, LA (2017) Feeding microalgae at a high level to finishing heifers increases the long-chain n-3 fatty acid composition of beef with only small effects on the sensory quality. International Journal of Food Science and Technology 53, 1405–1413.Google Scholar
Shingfield, KJ, Reynolds, CK, Hervás, G, Griinari, JM, Grandison, AS and Beever, DE (2006) Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. Journal of Dairy Science 89, 714–732.Google ScholarPubMed
Sinedino, LDP, Honda, PM, Souza, LRL, Lock, AL, Boland, MP, Staples, CR, Thatcher, WW and Santos, JEP (2017) Effects of supplementation with docosahexaenoic acid on reproduction of dairy cows. Reproduction 153, 707–723.Google ScholarPubMed
Sinclair, LA, Cooper, SL, Chikunya, S, Wilkinson, RG, Hallett, KG, Enser, M and Wood, JD (2005) Biohydrogenation of n-3 polyunsaturated fatty acids in the rumen and their effects on microbial metabolism and plasma fatty acid concentration in sheep. Animal Science 81, 239–248.Google Scholar
Till, BE, Huntington, JA, Posri, W, Early, R, Taylor-Pickard, J and Sinclair, LA (2019) Influence of rate of inclusion of microalgae on the sensory characteristics and fatty acid composition of cheese and performance of dairy cows. Journal of Dairy Science 102, 10934–10946.Google ScholarPubMed
Vanbergue, E, Peyraud, J and Hurtaud, C (2018) Effects of new n-3 fatty acid sources on milk fatty acid profile and milk fat properties in dairy cows. Journal of Dairy Research 85, 265–272.Google ScholarPubMed
Van Soest, PJ, Robertson, JB and Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Vlcek, M, Andrlikova, M, Barbato, O, Bina, V, Boland, MP, Dolezel, R, Lapatarova, M and Cech, S (2017) Supplementation of dairy cows with docosahexaenoic acid did not affect ovarian activity. Czech Journal of Animal Science 62, 457–465.Google Scholar
Zarate, R, El Jaber-Vazdekis, N, Tejera, N, Perez, JA and Rodriguez, C (2017) Significance of long chain polyunsaturated fatty acids in human health. Clinical and Translational Medicine 6, 25.Google ScholarPubMed
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