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Cone visual pigments of the Australian marsupials, the stripe-faced and fat-tailed dunnarts: Sequence and inferred spectral properties

Published online by Cambridge University Press:  05 April 2005

JESSICA STRACHAN ,
LING-YU E. CHANG ,
MATTHEW J. WAKEFIELD ,
JENNIFER A. MARSHALL GRAVES  and
SAMIR S. DEEB
JESSICA STRACHAN
Affiliation:
Departments of Medicine and Genome Sciences, University of Washington, Seattle
LING-YU E. CHANG
Affiliation:
Departments of Medicine and Genome Sciences, University of Washington, Seattle
MATTHEW J. WAKEFIELD
Affiliation:
Research School of Biological Sciences, The Australian National University, Canberra, Australia Centre for Bioinformation Science, JCSMR/MSI, The Australian National University, Canberra, Australia
JENNIFER A. MARSHALL GRAVES
Affiliation:
Research School of Biological Sciences, The Australian National University, Canberra, Australia
SAMIR S. DEEB
Affiliation:
Departments of Medicine and Genome Sciences, University of Washington, Seattle
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Abstract

Studies of color vision in marsupial mammals have been very limited. Two photoreceptor genes have been characterized from the tammar wallaby, but a third cone pigment was suggested by microspectrophotometric measurements on cone photoreceptors in two other species, including the fat-tailed dunnart, Sminthopsis crassicaudata. To determine the sequence and infer absorption maxima of the cone photoreceptor pigments of S. crassicaudata and the related stripe-faced dunnart (Sminthopsis macroura), we have used evolutionarily conserved sequences of the cone pigments of other species, including the tammar wallaby, to design primers to amplify the S. macroura and S. crassicaudata pigment sequences by the polymerase chain reaction (PCR) using genomic DNA or retinal cDNA as a template. These primers will be useful for amplifying cone opsin coding sequences from a variety of vertebrates. Amplified products were directly sequenced to determine gene structure and coding sequences. The inferred amino acid sequences of the cone visual pigments indicated that both species have middle-wave-sensitive (MWS) pigments with a predicted absorption maximum (λmax) at 530 nm, and ultraviolet-sensitive (UVS) pigments with a predicted λmax at 360 nm. The MWS pigments of the two species differ by two, and UVS by three amino acid positions. No evidence was obtained for a third cone pigment in either species.

Keywords

Cone opsinsMarsupialsDunnartSpectral tuning
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 3 , May 2004 , pp. 223 - 229
Copyright
© 2004 Cambridge University Press

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