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3 - Photosynthetic regulation
- Edited by Jaume Flexas, Universitat de les Illes Balears, Palma de Mallorca, Francesco Loreto, Hipólito Medrano, Universitat de les Illes Balears, Palma de Mallorca
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- Book:
- Terrestrial Photosynthesis in a Changing Environment
- Published online:
- 05 March 2013
- Print publication:
- 19 July 2012, pp 20-40
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Summary
Introduction
The evolution of oxygenic photosynthesis played an important role in the oxygenation of the atmosphere of the Earth. This rise in O2 also had an impact on the subsequent evolution of the photosynthetic organisms themselves, enabling them to develop more efficient bioenergetic systems. Thus, the reduction/oxidation (redox) reactions of the PET chain of green algae and higher plants and their regulation have become adapted to the O2-rich atmosphere of the Earth. Oxygen is, however, not only a product of photosynthesis but it is also a regulator of PET-chain activity and photosynthetic metabolism. Molecular O2 (3O2), is highly reactive and thus inherently toxic, but aerobic cells have evolved the ability to harness the energy potential of aerobic metabolism while minimising potentially harmful effects. Partly this is achieved by using the ROS, such as superoxide (O2–), hydrogen peroxide (H2O2) and singlet oxygen (1O2), formed as by-products of photosynthesis as important metabolic signals. The complex interactions of molecular O2 with the cellular electron-transport and metabolic systems of the cell have become an intrinsic feature of plant redox regulation and homeostasis.
Coordination between energy producing and energy utilising processes is at the heart of the processes that regulate photosynthesis and ensure efficient functioning over a wide range of environmental conditions. Respiration works alongside photosynthesis to secure efficient biological energy production in plant cells. However, unlike the regulation of respiration, which is driven by metabolic substrates that are protected from depletion by effective control mechanisms, the driving force for photosynthesis is the free energy of light, a substrate that cannot be conserved except through light harvesting, efficient charge separation and electron transport. The efficiency of the conversion of the free energy of light into chemical free energy by photosynthesis has been optimised during evolution. A complex network of defence systems protects photosynthesis against the potentially harmful effects of excess light, i.e., light capture that is in excess of the amount that can be used to drive photosynthesis. Efficient dissipation mechanisms are available to protect the photosynthetic membranes and their protein and pigment components by releasing the energy absorbed from light, largely as heat. Photosynthesis is thus able to operate in a highly flexible manner, harvesting energy efficiently at low irradiances and dissipating excess energy at high irradiance.
13 - Biochemical and molecular techniques for the study of photosynthetic processes
- Edited by Jaume Flexas, Universitat de les Illes Balears, Palma de Mallorca, Francesco Loreto, Hipólito Medrano, Universitat de les Illes Balears, Palma de Mallorca
-
- Book:
- Terrestrial Photosynthesis in a Changing Environment
- Published online:
- 05 March 2013
- Print publication:
- 19 July 2012, pp 186-205
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- Chapter
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Summary
Introduction
Increasing understanding of the many molecular and biochemical processes that respond in a purposive way to the changing environment has given rise to an appreciation that many, if not all, environmental cues evoke primary responses at a molecular level, and that it is these responses that result in changes in gross plant physiology and morphology. Likewise, changes in the relative proportions of metabolites and ions within intracellular compartments in response to such environmental cues also give rise to multiple changes in gene expression. The interaction between these levels of complexity in response to changes in the external environment is illustrated in Scheme 13.1.
This chapter describes and discusses approaches: (1) for the unbiased analyses of gene, protein and metabolite function facilitated by a variety of high-throughput approaches; and (2) for the focused analyses of specific genes, gene products and metabolites. The former approaches seek to identify hitherto unknown genes and molecular interactions, while the latter are used to probe those elements that we currently consider most important in understanding and interpreting how photosynthetic processes relate to ecophysiological questions. In particular, we discuss aspects of the isolation and assay of the carboxylating enzymes, Rubisco and phosphorenolpyruvate carboxylase (PEPC), owing to their pivotal roles in assimilation and to the continuing interest in their measurement. In general, we have selected methods and approaches that have been applied in our laboratories, but acknowledge that many alternative methods could have been described, which are equally reliable and quantitative.