2 results
The integration of cloning by nuclear transfer in the conservation of animal genetic resources
- D.N. Wells
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- Journal:
- BSAP Occasional Publication / Volume 30 / 2004
- Published online by Cambridge University Press:
- 27 February 2018, pp. 223-241
- Print publication:
- 2004
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Cloning mammals from somatic cells by nuclear transfer has the potential to assist with the preservation of genetic diversity. An increasing number of species have been successfully cloned by this approach; however, present methods are inefficient with few cloned embryos resulting in healthy offspring. In those livestock species that have already been cloned, it is clearly feasible to use cloning to preserve endangered breeds (e.g. the last surviving Enderby Island cow). The opportunity exists to recover oocytes from these cloned heifers and use frozen Enderby Island sperm from deceased bulls for in vitro fertilisation and thus, expand the genetic diversity of this breed. Where there exists an adequate understanding of the reproductive biology and embryology of the species concerned and adequate sources of females to supply both recipient oocytes and surrogates to gestate the pregnancies, intra-specific nuclear transfer and embryo transfer can be utilised. However, when these requirements cannot be met, as is common for most endangered species, cloning technology invariably involves the use of inter-species nuclear transfer and embryo transfer. Even in intra-specific cloning the source of oocyte for nuclear transfer is an important consideration. Typically, cloned animals are only genomic copies of the founder if they possess mitochondrial DNA which differs from the original animal. Different maternal lineages of oocytes both within and between breeds significantly affect cloning efficiency and livestock production characteristics. Cloning should not distract conservation efforts from encouraging the use of indigenous livestock breeds with traits of adaptation to local environments, the preservation of wildlife habitats or the use of other forms of assisted reproduction. Whilst it is often difficult to justify cloning in animal conservation at present, the appropriate cryo-preservation of tissues and cells from a wide selection of biodiversity is of paramount importance. This provides an insurance against further losses of genetic variation from dwindling populations, disease epidemics or even possible extinction. It would also complement the gene banking of gametes or embryos and can be performed more easily and cheaply. Future cloning from preserved somatic cells can reintroduce lost genes back into the breeding pool. With greater appreciation of the heritable attributes of traditional livestock breeds there is the desire to identify superior animals within these local populations and the genetic loci involved. Through clonal family performance testing, nuclear transfer can aid the selection of desirable genotypes and then the production of larger numbers of embryos or animals for natural breeding to more widely disseminate the desirable traits. With the identification of alleles conferring desirable attributes, transgenesis could be utilised to both improve traditional and industrial livestock breeds. This further emphasizes the importance of preserving global farm animal genetic resources.
Early embryo development in nuclear transfer clones derived from somatic cells of a high genetic merit dairy cow
- W.H. McMillan, D.N. Wells, AJ. Peterson, M.J. Donnison
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- Journal:
- BSAP Occasional Publication / Volume 26 / Issue 2 / September 2001
- Published online by Cambridge University Press:
- 27 February 2018, pp. 437-440
- Print publication:
- September 2001
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The reported post transfer survival rate to term of cloned embryos derived from both undifferentiated blastomeres and differentiated foetal and adult cells is very low (typically less than 20%). Furthermore, it is acknowledged that there are many technical issues that remain to be resolved to improve the efficiency of nuclear transfer before the technique will find widespread, practical and cost-effective use in multiplying valuable livestock in agriculture. The purpose of this study was to compare early embryo morphology following embryo transfer of nuclear transfer blastocysts derived from somatic cells of an adult Friesian cow with that of standard in vitro-produced embryos. In the present study, 150 embryos were transferred in bulk (i.e., 15, 20 or 25 per recipient) to the ipsilateral uterine horn of 8 recipients using standard non-surgical embryo transfer procedures. Embryos were then recovered following necropsy on either Day 14 or Day 23 of pregnancy and developmental traits described. Embryo recovery and elongation rates were similar on Day 14 of pregnancy (Table 1), although cloned conceptuses were longer and narrower (P<0.05). Embryo recovery and viability rates by Day 23 were similar, although many of the developmental traits appeared more advanced in cloned conceptuses. (Table 1). Allantois development was different because of greater widths and the presence of ‘spurs’ that were not observed with in vitro-produced embryos. We conclude that apparently abnormal conceptus development occurs by about 3 weeks of pregnancy following nuclear transfer, but that embryo survival is not compromised at this early stage of development.