We report a two-step method to rear three leafhopper species, Erythroneura elegantula Osborn, Erythroneura vitis (Harris), and Erythroneura ziczac Walsh, for experimental purposes on grapevines. The first step involves rearing adults on grapevines for reproduction, oviposition, and egg development. The second step involves rearing nymphs on isolated leaves placed in Petri dishes lined with agar. No mortality was observed and adults emerged in about three weeks. The three Erythroneura species were reared for 10 consecutive generations with minimal resources.