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Much effort has been focused on establishing optimal conditions for obtaining in vitro maturation of oocytes from different species with results comparable to those achieved after in vivo development (reviewed by Brackett, 1992). However, even though extraordinary progress has been made, the in vitro technology for oocyte maturation lags far behind that in vivo and improvements are needed to increase the quantity and quality of the embryos produced from these matured oocytes.
Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethyl-aminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.
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