Techniques for selective isolation, semiquantification and rapid
virulence
testing are described for Catenaria anguillulae, an important
endoparasitic fungus of nematodes. Selective isolation was achieved by
using second-stage juveniles of Anguina tritici as bait in soil
aliquots for 48 h. Semiquantitative estimation of the population of the
fungus was made by calculating the infection percentage of
inoculated juveniles. Rapid virulence testing was done by using second-stage
juveniles of Heterodera cajani, Meloidogyne incognita
and
A. tritici.