The probability of a Glossina pallidipes picking up an infection from a single blood meal in Lambwe Valley was estimated to be 0.0092 for Trypanosoma vivax, 0.0028 for T. congolense, 0.00097 for T. brucei and 0.00024 for the human infective T. brucei. In cattle, the prevalence of blood protozoa was Trypanosoma spp. 5.55%, Babesia spp. 2% and Theileria spp. 33.06%. Thirty-eight point three per cent of the cattle with trypanosomes also had Theileria spp., 2.12% of the cattle with trypanosomes also had Babesia spp. and 88.28% of the cattle with babesia had also Theileria spp.