The effect of pesticides on nitrogenase activity in rhizosphere soil from rice fields was investigated. The differential response of insecticides on nitrogenase depended on the method of field application. Results also showed that the differential response to the pesticides of. specific groups of nitrogen-fixing micro-organisms depended upon the method of application.
Soil incorporation of carbofuran stimulated the rhizosphere nitrogenase, while endosulfan and hexachlorocyclohexane inhibited it. Carbofuran and hexachlorocyclohexane stimulated nitrogenase when applied to the standing water. Seedling root dips of isofenphos stimulated nitrogenase, while endosulfan, BPMC and carbaryl showed a variable effect. Quinalphos inhibited nitrogenase irrespective of method of application.