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8 - Genetically engineered animals
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- By Carolina M. Maier, Department of Neurosurgery and Neurological Sciences Stanford University Medical School 1201 Welch Rd MSLS P357 Stanford, CA 94305 USA, Lilly Hsieh, Department of Neurosurgery and Neurological Sciences Stanford University Medical School 1201 Welch Rd MSLS P357 Stanford, CA 94305 USA, Pak H. Chan, Department of Neurosurgery and Neurosciences Stanford University Medical Center Palo Alto, CA 93304 USA
- Edited by Turgut Tatlisumak, Marc Fisher
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- Book:
- Handbook of Experimental Neurology
- Published online:
- 04 November 2009
- Print publication:
- 05 October 2006, pp 114-131
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- Chapter
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Summary
Introduction
Animal models that recreate specific pathogenic events and their corresponding behavioral outcomes are indispensable tools for exploring the underlying pathophysiologic mechanisms of disease and for investigating therapeutic strategies prior to testing them in human patients. Although rodents have a long tradition as models for human neurological diseases, they have received increasing attention in light of genetic engineering methods that have made it possible to create precisely defined genetic changes. The development of transgenic technology, a tool that allows sophisticated manipulation of the genome, has provided an unprecedented opportunity to expand our understanding of many aspects of neuronal development, function, and disease.
Transgenic animals are specific variants of species following the introduction and/or integration of a new gene or genes into the genome of the host animal. Transgenic technology is now routinely used to increase the level of (overexpress) particular proteins or enzymes in animals or to mutate or inactivate a particular gene using a “knockout” approach. One of the most commonly used animals in transgenic techniques is the mouse, a species in which transgene microinjections into the pronuclei of fertilized oocytes and the subsequent expression of the transgene in the animal have been carefully worked out.
Since their development in the 1980s, the transgenic and knockout technologies have allowed us to examine the regulation of gene expression and the pathophysiology of its alterations.