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The use of biochemical tests to identify multiple insecticide resistance mechanisms in field-selected populations of Anopheles subpictus Grassi (Diptera: Culicidae)

Published online by Cambridge University Press:  10 July 2009

J. Hemingway ,
K. G. I. Jayawardena ,
I. Weerasinghe  and
P. R. J. Herath
J. Hemingway*
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
K. G. I. Jayawardena
Affiliation:
Anti-Malaria Campaign, P.O. Box 1472, Narahenpita, Colombo 5, Sri Lanka
I. Weerasinghe
Affiliation:
Anti-Malaria Campaign, P.O. Box 1472, Narahenpita, Colombo 5, Sri Lanka
P. R. J. Herath
Affiliation:
Anti-Malaria Campaign, P.O. Box 1472, Narahenpita, Colombo 5, Sri Lanka
*
* To whom all correspondence should be sent.
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Abstract

Anopheles subpictus Grassi in Sri Lanka is under selection pressure from both agricultural and public health insecticides. Agricultural selection pressure has produced larval specific carbamate resistance which appears to be correlated with high esterase activity. High esterase activity was found in both larvae and adults, but one of the larval elevated bands was not present in the adult, and two other adult bands were not found in the larvae. Broad spectrum organophosphate resistance was found in both the larvae and the adults and was associated with an increase in mixed-function oxidase activity. There was no evidence of an altered AChE mechanism in this population.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 77 , Issue 1 , March 1987 , pp. 57 - 66
Copyright
Copyright © Cambridge University Press 1987

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References

Ayad, H. & Georghiou, G. P. (1975). Resistance to organophosphates and carbamates in Anopheles albimanus based on reduced sensitivity of acetylcholinesterase.—J. econ. Ent. 68, 295–297.CrossRefGoogle ScholarPubMed
Devonshire, A. L. & Sawicki, R. M. (1979). Insecticide-resistant Myzus persicae as an example of evolution by gene duplication.—Nature, Lond. 280, 140–141.CrossRefGoogle Scholar
Georghiou, G. P., Pasteur, N. & Hawley, M. K. (1980). Linkage relationships between organophosphate resistance and a highly active esterase-B in Culex quinquefasciatus from California.—J. econ. ent. 73, 301–305.CrossRefGoogle Scholar
Hemingway, J. (1982). The biochemical nature of malathion resistance in Anopheles stephensi from Pakistan.—Pestic. Biochem. & Physiol. 17, 149–155.CrossRefGoogle Scholar
Hemingway, J. (1983). Biochemical studies on malathion resistance in Anopheles arabiensis from Sudan.—Trans. R. Soc. trop. Med. Hyg. 77, 477–480.CrossRefGoogle ScholarPubMed
Hemingway, J. (1985). Malathion carboxylesterase enzymes in Anopheles arabiensis from Sudan.—Pestic. Biochem. & Physiol. 23, 309–313.CrossRefGoogle Scholar
Hemingway, J. & Georghiou, G. P. (1983). Studies on the acetylcholinesterase of Anopheles albimanus resistant and susceptible to organophosphate and carbamate insecticides.—Pestic. Biochem. & Physiol. 19, 167–171.CrossRefGoogle Scholar
Hemingway, J. & Georghiou, G. P. (1984). Baseline esterase levels for anopheline and culicine mosquitoes.—Mosquito News 44, 33–35.Google Scholar
Hemingway, J., Jayawardena, K. G. I. & Herath, P. R. J. (in press). Pesticide resistance mechanisms produced by the field selection pressures on Anopheles nigerrimus and Anopheles culicifacies in Sri Lanka.—Bull. Wld Hlth Org.Google Scholar
Hemingway, J., Smith, C., Jayawardena, K. G. I. & Herath, P. R. J. (1986). Field and laboratory detection of the altered acetylcholinesterase resistance genes which confer organophosphate and carbamate resistance in mosquitoes (Diptera: Culicidae).—Bull. ent. Res. 76, 559–565.CrossRefGoogle Scholar
Herath, P. R. J. & Joshi, G. P. (1986). The role of agricultural and public health pesticide use in the development of insecticide resistance in Anopheles subpictus in Sri Lanka: comparison with two other Sri Lankan anophelines.—Trans. R. Soc. trop. Med. Hyg. 80, 649–652.CrossRefGoogle Scholar
Kulkarni, A. P. & Hodgson, E. (1976). Spectral interactions of insecticide synergists with microsomal cytochrome P-450 from insecticide-resistant and susceptible house flies.—Pestic. Biochem. & Physiol. 6, 183–191.CrossRefGoogle Scholar
Lines, J. D., Ahmed, M. A. E. & Curtis, C. F. (1984). Genetic studies of malathion resistance in Anopheles arabiensis Patton (Diptera: Culicidae).—Bull. ent. Res. 74, 317–325.CrossRefGoogle Scholar
Matthews, W. A. (1980). The metabolism of malathion in vivo by two strains of Rhyzopertha dominica (F.), the lesser grain borer.—Pestic. Biochem. & Physiol. 13, 303–312.CrossRefGoogle Scholar
Pasteur, N. & Georghiou, G. P. (1981). Filter paper test for rapid determination of phenotypes with high esterase activity in organophosphate resistant mosquitoes.—Mosquito News 41, 181–183.Google Scholar
Villani, F., White, G. B., Curtis, C. F. & Miles, S. J. (1983). Inheritance and activity of some esterases associated with organophosphate resistance in mosquitoes of the complex of Culex pipiens L. (Diptera: Culicidae).—Bull. ent. Res. 73, 153–170.CrossRefGoogle Scholar