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Carbohydrase activity in the pancreatic tissue and small intestine mucosa of sheep fed dried-grass or ground maize-based diets

Published online by Cambridge University Press:  27 March 2009

A. N. Janes ,
T. E. C. Weekes  and
D. G. Armstrong
A. N. Janes
Affiliation:
Department of Agricultural Biochemistry and Nutrition, The University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU
T. E. C. Weekes
Affiliation:
Department of Agricultural Biochemistry and Nutrition, The University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU
D. G. Armstrong
Affiliation:
Department of Agricultural Biochemistry and Nutrition, The University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU
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Summary

Two groups of six sheep were fed either dried-grass or ground maize-based diets for at least 4 weeks before slaughter. Samples of the small intestinal mucosa and spancreatic tissue were assayed for a-amylase, glucoamylase, maltase and oligo-l,6-glucosidase.

The pancreatic tissue contained high activities of α-amylase and much lower activities of glucoamylase, maltase and oligo-1,6-glucosidase. There was no effect of diet on the specific activities of any of these enzymes in the pancreatic tissue.

The activity of α-amylase adsorbed on to the mucosa of the small intestine was greatest in the proximal region of the small intestine, the activity generally declining with increasing distance away from the pylorus. There was no diet effect on the absorbed α-amylase activity.

Similar patterns of distribution along the small intestine were observed for maltase, glucoamylase and oligo-1,6-glucosidase with the highest activities in t he jejunum. There was no overall effect of diet on glucoamylase or maltase specific activities and glucoamylase total activity, although the total activities of maltase and oligo-1,6-glucosidase were significantly greater for the sheep fed the ground maize-based diet (P < 0·05).

It is suggested that ruminant animals may be capable of digesting large amounts of starch in the small intestine through an adaptation in the activity of the host carbohydrases.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 104 , Issue 2 , April 1985 , pp. 435 - 443
Copyright
Copyright © Cambridge University Press 1985

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