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Comparative histochemical studies of glycosidase activity in some helminths

Published online by Cambridge University Press:  18 November 2009

T. Fujino  and
Y. Ishii
T. Fujino
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan.
Y. Ishii
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan.
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Abstract

Comparative histochemical studies of glycosidase activity were carried out in Clonorchis sinensis, Eurytrema pancreaticum, Fasciola hepatica, Dipylidium caninum, Hymenolepis nana, Ascaris suum, Toxocara canis, Ancylostoma caninum, Trichuris vulpis and Dirofilaria immitis. The enzymes examined were: N-Acetyl-α-Glucosaminidase (Ec 3.2.1.30), α-Glucuronidase (Ec 3.2.1.31) and α-Galactosidase (Ec 3.2.1.23). There were variations in enzyme distribution and intensity among the species and also between trematodes and nematodes; no marked positive reaction for these enzymes occurred in cestodes. In some trematodes, the caeca, especially in the brush border, and the tegument, subtegumental cells and testes, were reactive to the enzymes. In nematodes, although there was variation in reactions among species, N-acetyl-α-glucosaminidase and α-galactosidase were localized in the hypodermis and lateral cords excluding the excretory canal, and coelomocytes, intestinal epithelium and the walls of the reproductive systems.

Keywords

histochemistryglcosidasestrematodescestodesnematodes
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 1 , March 1986 , pp. 1 - 13
Copyright
Copyright © Cambridge University Press 1986

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