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Duddingtonia flagrans chlamydospores in nutritional pellets: effect of storage time and conditions on the trapping ability against Haemonchus contortus larvae

Published online by Cambridge University Press:  16 August 2013

J.A. Fitz-Aranda ,
P. Mendoza-de-Gives ,
J.F.J. Torres-Acosta ,
E. Liébano-Hernández ,
M.E. López-Arellano ,
C.A. Sandoval-Castro  and
H. Quiroz-Romero
J.A. Fitz-Aranda
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
P. Mendoza-de-Gives*
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
J.F.J. Torres-Acosta*
Affiliation:
FMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, México
E. Liébano-Hernández
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
M.E. López-Arellano
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla, No. 8534, Col Progreso, Jiutepec, Morelos, México
C.A. Sandoval-Castro
Affiliation:
FMVZ, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, México
H. Quiroz-Romero
Affiliation:
FMVZ, Universidad Nacional Autónoma de México. Circuito Exterior, Ciudad Universitaria, Coyoacán, México, D.F.
*
*E-mail: pedromdgives@yahoo.com and tacosta@uady.mx
*E-mail: pedromdgives@yahoo.com and tacosta@uady.mx
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Abstract

The study evaluated the effect of storage time and conditions of nutritional pellets (NP) containing Duddingtonia flagrans chlamydospores on its in vitro trapping ability against Haemonchus contortus L3 larvae. The treated batch (200 NP) contained 4 ×  106 chlamydospores of the FTH0-8 strain, whereas the control batch (200 NP) was produced without spores. Both NP batches were exposed to four experimental storage conditions: (T1) shelves (indoors); (T2) refrigeration (4°C); (T3) outdoors under a roof; and (T4) 100% outdoors. Each group comprised 48 NP with spores and 48 NP without spores (control). The ability of D. flagrans spores to trap H. contortus L3 larvae was evaluated for 8 weeks for each storage condition. For that purpose, six randomly selected NP with spores were compared to their respective control NP. Each NP was individually crushed. The crushed material (1 g) was placed on the surface of a 2% water agar plate with 200 H. contortus L3 larvae. Plates were sealed and were incubated at room temperature for 8 days. The whole content of every plate was transferred to a Baermann apparatus to recover the remaining larvae. There was a clear larval reduction in the NP with spores, compared to the respective control NP in the four storage conditions (P< 0.05). The mean reductions ( ± SEM) of the storage conditions were 67 ± 4.9 (T2), 77 ± 6.1 (T1), 81.5 ± 3.8 (T4) and 82.1 ± 2.5 (T3). Larval reductions were similar at all times and were not affected by storage conditions or storage time (R2< 0.2; P>0.05). The long-term shelf-life of the chlamydospores in the NP suggests that this spore dosage technology is a viable option.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 1 , January 2015 , pp. 13 - 18
Copyright
Copyright © Cambridge University Press 2013 

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