Hostname: page-component-76fb5796d-2lccl Total loading time: 0 Render date: 2024-04-26T04:52:57.630Z Has data issue: false hasContentIssue false

The effect of soaking injury in bean seeds on protein synthesis in embryonic axes

Published online by Cambridge University Press:  19 September 2008

Johan C. Pretorius
Affiliation:
Department of Botany and Genetics, University of the Orange Free State, PO Box 339, Bloemfontein, 9300, South Africa
J. G. Chris Small*
Affiliation:
Department of Botany and Genetics, University of the Orange Free State, PO Box 339, Bloemfontein, 9300, South Africa
*
* Correspondence

Abstract

The capacity for protein synthesis in embryonic axes of Phaseolus vulgaris L. cv. Top Crop seeds was reduced markedly by soaking in airsaturated and CO2-saturated water. The former treatment induces soaking injury while the latter treatment prevents injury. After soaking, the capacity for protein synthesis in axes of seeds soaked in air-saturated water declined and the pattern of synthesis remained almost similar to that at the time of termination of soaking. However, the capacity and pattern of protein synthesis in axes of seeds soaked in CO2-saturated water recovered subsequent to soaking and approached that of non-soaked seeds.

Type
Short Communication
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

1

Present address: Faculty of Applied Sciences, Technikon O.F.S., Private Bag X20539, Bloemfontein 9300, South Africa.

References

Barton, L.V. (1950) Relation of different gases to the soaking injury of seeds. Contributions of the Boyce Thompson Institute 16, 5571.Google Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248254.CrossRefGoogle ScholarPubMed
Dasgupta, J. and Bewley, J.D. (1982) Desiccation of axes of Phaseolus vulgaris during development of a switch from a development pattern of protein synthesis to a germination pattern. Plant Physiology 70, 12241227.CrossRefGoogle ScholarPubMed
Fish, L.E. and Jagendorf, A.T. (1982) High rates of protein synthesis by isolated chloroplasts. Plant Physiology 70, 11071114.CrossRefGoogle ScholarPubMed
Kermode, A.R. and Bewley, J.D. (1986) The role of maturation drying in the transition from seed development to germination. Journal of Experimental Botany 37, 18871898.CrossRefGoogle Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.CrossRefGoogle ScholarPubMed
Matters, G.L. and Scandalios, J.G. (1986) Changes in plant gene expression during stress. Developmental Genetics 7, 167175.CrossRefGoogle ScholarPubMed
Orphanos, P.I. and Heydecker, W. (1968) On the nature of the soaking injury of Phaseolus vulgaris seeds. Journal of Experimental Botany 19, 770784.CrossRefGoogle Scholar
Small, J.G.C., Botha, F.C., Pretorius, J.C. and Hoffman, E. (1991) Evidence for an ethylene requirement to reduce soaking injury in bean seeds and the beneficial effect of heavy metals. Journal of Experimental Botany 42, 277280.CrossRefGoogle Scholar