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Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles

Published online by Cambridge University Press:  25 November 2020

Li Ang ,
Cao Haixia ,
Li Hongxia ,
Li Ruijiao ,
Guo Xingping  and
Wang Huaixiu Open the ORCID record for Wang Huaixiu [Opens in a new window]
Li Ang
Affiliation:
Department of Biochemistry, Shanxi Medical University, Taiyuan030001, China
Cao Haixia
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Li Hongxia
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Li Ruijiao
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Guo Xingping
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Wang Huaixiu*
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China Beijing Perfect Family Hospital, Beijing, 100034, China
*
Author for correspondence: Wang Huaixiu. Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China. E-mail: 976378008@qq.com
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Summary

The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.

Keywords

C-type natriuretic peptideMiceIn vitro fertilizationIn vitro growthOvarian follicle cultureOocyte
Type
Research Article
Information
Zygote , Volume 29 , Issue 2 , April 2021 , pp. 150 - 154
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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