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New Concept in Helium Cooled Transmission Microscopes Philips Tecnai F30 Helium: Design and First Results

Published online by Cambridge University Press:  02 July 2020

U. Lücken ,
R. Wagner ,
AF de Jong  and
W. Hax
U. Lücken
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
R. Wagner
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
AF de Jong
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
W. Hax
Affiliation:
FEI Electron Optics, PO Box 218, 5600, MD, Eindhoven, The Netherlands
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Abstract

To date it has been possible to solve the structure of several membrane proteins to atomic resolution with cryo-electron crystallography (1-4). in all studies two-dimensional crystals were required as well as special provisions on the transmission microscope. in all cases a field emission gun provided the necessary coherence and liquid-Helium cooling the specimen protection (4,5).

Although repeatedly studies have been done on the cryo-protection factor of beam sensitive specimen at liquid-Nitrogen and liquid-Helium temperatures (6-10), the data available are still too limited for a clear demonstration of the superiority of liquid-Helium. Especially investigations are needed with individual particles embedded in amorphous ice (11).

To meet the increasing demand for liquid-Helium cooling, FEI developed the Tecnai F30 Helium. The challenge was to provide an instrument with a very stable cryo-stage, which can work at liquid- Nitrogen as well as liquid-Helium temperature.

Type
Corporate Session (Organized by M. Kersker)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 868 - 869
Copyright
Copyright © Microscopy Society of America 2001

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References

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