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Expression and Co-Localization of C-Type Natriuretic Peptide and Endothelin in Human Kidney

Published online by Cambridge University Press:  02 July 2020

C. Wei  and
J. C. Burnett Jr.
C. Wei
Affiliation:
, Cardiorenal Molecular Research, Departments of Surgery, University of Maryland School of Medicine, Baltimore, MD, 21201
J. C. Burnett Jr.
Affiliation:
Department of Medicine, Mayo Clinic, Rochester, MN, 55905
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Extract

C-type natriuretic peptide (CNP) is an endothelium-drived vasorelaxing factor which is present in human plasma, urine and renal epithelial cells. Endothelin (ET) is a potent renal and systemic vasoconstrictor and sodium regulating peptide. To date, the localization of C-type natriuretic peptide and endothelin gene expression in human kidney remains poorly defined.

We determined the co-localization of C-type natriuretic peptide and endothelin mRNA in normal human kidney using in situ hybridization (ISH) with synthesized human C-type natriuretic peptide or human endothelin oligonucleotide probes together with immunohistochemical staining (IHCS) with human CNP-22 and ET-1 antibodies with no crossreactivity. The sections treated with nonimmune rabbit serum (NRS) demonstrated no immunoperoxidase activity. Human renal tissue (n=7) was obtained from biopsy of normal cadaveric donor kidneys and normal autopsy specimens.

In situ hybridization (Figure 1) demonstrated that C-type natriuretic peptide and endothelin mRNA presence in human kidney which was localized to proximal and distal tubes with similar intensities.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 600 - 601
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Borgeson, D. D., et al. Clin Sci (1998) 95, 195–202.CrossRefGoogle Scholar
2.Brenner, D., Gerstberger, R.. Endocrinology (1999) 140, 1622–1629.CrossRefGoogle Scholar
3.Lai, K. N., et al. J Hypertens (1999) 17, 575–583.CrossRefGoogle Scholar
4.Wihelm, S. M., et al. Kidney Int (1999) 55, 1011–1018CrossRefGoogle Scholar
5.Ruschitzka, R., et al. J Am Soc Nephrol (1999) 10, 953–962Google Scholar
6.Choukroun, G., et al. J Clin Invest (1998) 102, 1311–1320.CrossRefGoogle Scholar
5. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHA-MD, NKF and University of Maryland School of Medicine.Google Scholar